Cloning, Purification, and Characterization of a Thermostable β-Glucosidase from Thermotoga thermarum DSM 5069
A 56-kDa β-glucosidase (TthBgl) derived from Thermotoga thermarum DSM 5069 was expressed and purified from Escherichia coli BL21 (DE3). The purified enzyme showed hydrolytic activity towards only p-nitrophenyl-β-D-glucopyranoside among the synthetic glycosides tested. The pH maximum was 5.0, and und...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2016-02-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_2_3165_Long_Cloning_Purification_Characterization_Glucosidase |