Cloning, Purification, and Characterization of a Thermostable β-Glucosidase from Thermotoga thermarum DSM 5069

A 56-kDa β-glucosidase (TthBgl) derived from Thermotoga thermarum DSM 5069 was expressed and purified from Escherichia coli BL21 (DE3). The purified enzyme showed hydrolytic activity towards only p-nitrophenyl-β-D-glucopyranoside among the synthetic glycosides tested. The pH maximum was 5.0, and und...

Повний опис

Бібліографічні деталі
Автори: Lingfeng Long, Hao Shi, Xun Li, Yu Zhang, Jinguang Hu, Fei Wang
Формат: Стаття
Мова:English
Опубліковано: North Carolina State University 2016-02-01
Серія:BioResources
Предмети:
Онлайн доступ:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_2_3165_Long_Cloning_Purification_Characterization_Glucosidase