Primer and platform effects on 16S rRNA tag sequencing

Primer and platform effects on 16S rRNA tag sequencing

Sequencing of 16S rRNA gene tags is a popular method for profiling and comparing microbial communities. The protocols and methods used, however, vary considerably with regard to amplification primers, sequencing primers, sequencing technologies; as well as quality filtering and clustering. How resul...

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Bibliographic Details
Main Authors: Julien eTremblay, Kanwar eSingh, Alison eFern, Edward S Kirton, Shaomei eHe, Tanja eWoyke, Janey eLee, Feng eChen, Jeffery L Dangl, Susannah G Tringe
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-08-01
Series:Frontiers in Microbiology
Subjects:
Amplification
community assembly
Microbial Diversity
Microbial Population and Community Ecology
16S rRNA gene sequencing
high throughput sequencing
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00771/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00771/full

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