A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Abstract Background Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants have helped elucidate the genetic ba...

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Bibliographic Details
Main Authors: Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman, James V. Moroney
Format: Article
Language:English
Published: BMC 2017-04-01
Series:Plant Methods
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13007-017-0170-x