Optimization and evaluation of viral metagenomic amplification and sequencing procedures toward a genome-level resolution of the human fecal DNA virome

Optimization and evaluation of viral metagenomic amplification and sequencing procedures toward a genome-level resolution of the human fecal DNA virome

Introduction: Viruses in the human gut have been linked to health and disease. Deciphering the gut virome is dependent on metagenomic sequencing of the virus-like particles (VLPs) purified from the fecal specimens. A major limitation of conventional viral metagenomic sequencing is the low recoverabi...

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Bibliographic Details
Main Authors: Guangyang Wang, Shenghui Li, Qiulong Yan, Ruochun Guo, Yue Zhang, Fang Chen, Xiangge Tian, Qingbo Lv, Hao Jin, Xiaochi Ma, Yufang Ma
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Journal of Advanced Research
Subjects:
Fecal DNA virome
Virus-like particles
Optimal method
High-quality viral genome
Nanopore sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2090123222001928

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http://www.sciencedirect.com/science/article/pii/S2090123222001928

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