Anion-Exchange Chromatography at the Service of Gene Therapy: Baseline Separation of Full/Empty Adeno-Associated Virus Capsids by Screening of Conditions and Step Gradient Elution Mode

Anion-Exchange Chromatography at the Service of Gene Therapy: Baseline Separation of Full/Empty Adeno-Associated Virus Capsids by Screening of Conditions and Step Gradient Elution Mode

Gene therapy is opening unprecedented opportunities for novel therapeutic approaches. Based on the concept of rescuing function mutations by co-expressing the correct gene to allow biological functions to be restored, it requires the use of viral vectors to ensure the proper delivery of therapeutic...

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Bibliographic Details
Main Authors: Megane K. Aebischer, Hugo Gizardin-Fredon, Honorine Lardeux, Dominik Kochardt, Carsten Elger, Markus Haindl, Raphael Ruppert, Davy Guillarme, Valentina D’Atri
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:International Journal of Molecular Sciences
Subjects:
anion-exchange chromatography
step gradient
gene therapy
recombinant adeno-associated virus
full/empty ratio
Online Access:https://www.mdpi.com/1422-0067/23/20/12332

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https://www.mdpi.com/1422-0067/23/20/12332

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