Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction
<p>Abstract</p> <p>Background</p> <p>Generalized hypoxic pulmonary vasoconstriction (HPV) occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. Th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-06-01
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| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/11/1/81 |
| _version_ | 1811311094116909056 |
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| author | Surber Mark W Hoglen Niel C Elliott Garry T Dahal Bhola K Schermuly Ralph T Egemnazarov Bakytbek Weissmann Norbert Grimminger Friedrich Seeger Werner Ghofrani Hossein A |
| author_facet | Surber Mark W Hoglen Niel C Elliott Garry T Dahal Bhola K Schermuly Ralph T Egemnazarov Bakytbek Weissmann Norbert Grimminger Friedrich Seeger Werner Ghofrani Hossein A |
| author_sort | Surber Mark W |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Generalized hypoxic pulmonary vasoconstriction (HPV) occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. The aim of this project was to investigate and compare the effects of nebulization of nitrite and different formulations of acidified sodium nitrite on acute HPV.</p> <p>Methods</p> <p><it>Ex vivo </it>isolated rabbit lungs perfused with erythrocytes in Krebs-Henseleit buffer (adjusted to 10% hematocrit) and <it>in vivo </it>anesthetized catheterized rabbits were challenged with periods of hypoxic ventilation alternating with periods of normoxic ventilation. After baseline hypoxic challenges, vehicle, sodium nitrite or acidified sodium nitrite was delivered via nebulization. In the <it>ex vivo </it>model, pulmonary arterial pressure and nitric oxide concentrations in exhaled gas were monitored. Nitrite and nitrite/nitrate were measured in samples of perfusion buffer. Pulmonary arterial pressure, systemic arterial pressure, cardiac output and blood gases were monitored in the <it>in vivo </it>model.</p> <p>Results</p> <p>In the <it>ex vivo </it>model, nitrite nebulization attenuated HPV and increased nitric oxide concentrations in exhaled gas and nitrite concentrations in the perfusate. The acidified forms of sodium nitrite induced higher levels of nitric oxide in exhaled gas and had longer vasodilating effects compared to nitrite alone. All nitrite formulations increased concentrations of circulating nitrite to the same degree. In the <it>in vivo </it>model, inhaled nitrite inhibited HPV, while pulmonary arterial pressure, cardiac output and blood gases were not affected. All nitrite formulations had similar potency to inhibit HPV. The tested concentration of appeared tolerable.</p> <p>Conclusion</p> <p>Nitrite alone and in acidified forms effectively and similarly attenuates HPV. However, acidified nitrite formulations induce a more pronounced increase in nitric oxide exhalation.</p> |
| first_indexed | 2024-04-13T10:11:47Z |
| format | Article |
| id | doaj.art-5c959187859e4d01b92bc6b4bbda4ca4 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-13T10:11:47Z |
| publishDate | 2010-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-5c959187859e4d01b92bc6b4bbda4ca42022-12-22T02:50:52ZengBMCRespiratory Research1465-99212010-06-011118110.1186/1465-9921-11-81Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstrictionSurber Mark WHoglen Niel CElliott Garry TDahal Bhola KSchermuly Ralph TEgemnazarov BakytbekWeissmann NorbertGrimminger FriedrichSeeger WernerGhofrani Hossein A<p>Abstract</p> <p>Background</p> <p>Generalized hypoxic pulmonary vasoconstriction (HPV) occurring during exposure to hypoxia is a detrimental process resulting in an increase in lung vascular resistance. Nebulization of sodium nitrite has been shown to inhibit HPV. The aim of this project was to investigate and compare the effects of nebulization of nitrite and different formulations of acidified sodium nitrite on acute HPV.</p> <p>Methods</p> <p><it>Ex vivo </it>isolated rabbit lungs perfused with erythrocytes in Krebs-Henseleit buffer (adjusted to 10% hematocrit) and <it>in vivo </it>anesthetized catheterized rabbits were challenged with periods of hypoxic ventilation alternating with periods of normoxic ventilation. After baseline hypoxic challenges, vehicle, sodium nitrite or acidified sodium nitrite was delivered via nebulization. In the <it>ex vivo </it>model, pulmonary arterial pressure and nitric oxide concentrations in exhaled gas were monitored. Nitrite and nitrite/nitrate were measured in samples of perfusion buffer. Pulmonary arterial pressure, systemic arterial pressure, cardiac output and blood gases were monitored in the <it>in vivo </it>model.</p> <p>Results</p> <p>In the <it>ex vivo </it>model, nitrite nebulization attenuated HPV and increased nitric oxide concentrations in exhaled gas and nitrite concentrations in the perfusate. The acidified forms of sodium nitrite induced higher levels of nitric oxide in exhaled gas and had longer vasodilating effects compared to nitrite alone. All nitrite formulations increased concentrations of circulating nitrite to the same degree. In the <it>in vivo </it>model, inhaled nitrite inhibited HPV, while pulmonary arterial pressure, cardiac output and blood gases were not affected. All nitrite formulations had similar potency to inhibit HPV. The tested concentration of appeared tolerable.</p> <p>Conclusion</p> <p>Nitrite alone and in acidified forms effectively and similarly attenuates HPV. However, acidified nitrite formulations induce a more pronounced increase in nitric oxide exhalation.</p>http://respiratory-research.com/content/11/1/81 |
| spellingShingle | Surber Mark W Hoglen Niel C Elliott Garry T Dahal Bhola K Schermuly Ralph T Egemnazarov Bakytbek Weissmann Norbert Grimminger Friedrich Seeger Werner Ghofrani Hossein A Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction Respiratory Research |
| title | Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction |
| title_full | Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction |
| title_fullStr | Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction |
| title_full_unstemmed | Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction |
| title_short | Nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction |
| title_sort | nebulization of the acidified sodium nitrite formulation attenuates acute hypoxic pulmonary vasoconstriction |
| url | http://respiratory-research.com/content/11/1/81 |
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