Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureus
Objective: To study the antibacterial activity and stability of polysaccharides from Meitan White Tea against standard strains of Staphylococcus aureus in vitro. Method: The minimum inhibitory concentration (MIC) of polysaccharides from Meitan White Tea on Staphylococcus aureus was determined by oxf...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-02-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050041 |
| _version_ | 1828093917969514496 |
|---|---|
| author | Shanshan SONG Aihua YANG Xiaomin WANG Wan LONG Weiwei WANG |
| author_facet | Shanshan SONG Aihua YANG Xiaomin WANG Wan LONG Weiwei WANG |
| author_sort | Shanshan SONG |
| collection | DOAJ |
| description | Objective: To study the antibacterial activity and stability of polysaccharides from Meitan White Tea against standard strains of Staphylococcus aureus in vitro. Method: The minimum inhibitory concentration (MIC) of polysaccharides from Meitan White Tea on Staphylococcus aureus was determined by oxford cup test and microdilution broth method. The effects of temperature, pH, ultraviolet and metal ion on the antibacterial activity of polysaccharides from Meitan White Tea were studied. Results: The polysaccharides from Meitan White Tea had significant antibacterial activity against Staphylococcus aureus, and the minimum inhibitory concentration was 8 mg/mL. The bacteriostatic stability experiment of MIC was carried out, and the OD600 value of Staphylococcus aureus was used as an index. The results showed that the antibacterial activity of polysaccharides from Meitan White Tea was stable at 20~60 ℃, but it decreased slightly after being treated at high temperature (80~100 ℃), about 45.8% retained at high temperature compared with 4 ℃. The antibacterial activity remained stable at pH4~10. After 30 W ultraviolet treatment for 10~50 min, the antibacterial activity had no significant change (P>0.05). Under the treatment of 0.1 mol/L Na+ and K+, the antibacterial activity was stable, but under the treatment of 0.1 mol/L Ca2+, the antibacterial activity was lost. Conclusion: The polysaccharides from Meitan White Tea have stable inhibitory activity against Staphylococcus aureus in vitro, which provides a basis for its development as a natural preservative. |
| first_indexed | 2024-04-11T06:52:23Z |
| format | Article |
| id | doaj.art-5c96149424b24725a9d8731242c02b59 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T06:52:23Z |
| publishDate | 2022-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-5c96149424b24725a9d8731242c02b592022-12-22T04:39:09ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-0143311411910.13386/j.issn1002-0306.20210500412021050041-3Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureusShanshan SONG0Aihua YANG1Xiaomin WANG2Wan LONG3Weiwei WANG4College of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, ChinaCollege of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, ChinaCollege of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, ChinaCollege of Pharmacy, Zunyi Medical University, Zunyi 563000, ChinaCollege of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, ChinaObjective: To study the antibacterial activity and stability of polysaccharides from Meitan White Tea against standard strains of Staphylococcus aureus in vitro. Method: The minimum inhibitory concentration (MIC) of polysaccharides from Meitan White Tea on Staphylococcus aureus was determined by oxford cup test and microdilution broth method. The effects of temperature, pH, ultraviolet and metal ion on the antibacterial activity of polysaccharides from Meitan White Tea were studied. Results: The polysaccharides from Meitan White Tea had significant antibacterial activity against Staphylococcus aureus, and the minimum inhibitory concentration was 8 mg/mL. The bacteriostatic stability experiment of MIC was carried out, and the OD600 value of Staphylococcus aureus was used as an index. The results showed that the antibacterial activity of polysaccharides from Meitan White Tea was stable at 20~60 ℃, but it decreased slightly after being treated at high temperature (80~100 ℃), about 45.8% retained at high temperature compared with 4 ℃. The antibacterial activity remained stable at pH4~10. After 30 W ultraviolet treatment for 10~50 min, the antibacterial activity had no significant change (P>0.05). Under the treatment of 0.1 mol/L Na+ and K+, the antibacterial activity was stable, but under the treatment of 0.1 mol/L Ca2+, the antibacterial activity was lost. Conclusion: The polysaccharides from Meitan White Tea have stable inhibitory activity against Staphylococcus aureus in vitro, which provides a basis for its development as a natural preservative.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050041meitan white teatea polysaccharidesstaphylococcus aureusantibacterial activitystability |
| spellingShingle | Shanshan SONG Aihua YANG Xiaomin WANG Wan LONG Weiwei WANG Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureus Shipin gongye ke-ji meitan white tea tea polysaccharides staphylococcus aureus antibacterial activity stability |
| title | Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureus |
| title_full | Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureus |
| title_fullStr | Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureus |
| title_full_unstemmed | Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureus |
| title_short | Antibacterial Activity and Stability of Polysaccharides from Meitan White Tea on Staphylococcus aureus |
| title_sort | antibacterial activity and stability of polysaccharides from meitan white tea on staphylococcus aureus |
| topic | meitan white tea tea polysaccharides staphylococcus aureus antibacterial activity stability |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021050041 |
| work_keys_str_mv | AT shanshansong antibacterialactivityandstabilityofpolysaccharidesfrommeitanwhiteteaonstaphylococcusaureus AT aihuayang antibacterialactivityandstabilityofpolysaccharidesfrommeitanwhiteteaonstaphylococcusaureus AT xiaominwang antibacterialactivityandstabilityofpolysaccharidesfrommeitanwhiteteaonstaphylococcusaureus AT wanlong antibacterialactivityandstabilityofpolysaccharidesfrommeitanwhiteteaonstaphylococcusaureus AT weiweiwang antibacterialactivityandstabilityofpolysaccharidesfrommeitanwhiteteaonstaphylococcusaureus |