Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model
ABSTRACT: The objective of this study was to apply microwave-assisted extraction using green solvents starting from 3 different wine (white, rosé, and red) lees and to test their bio-response on sheep peripheral blood mononuclear cells proliferation, Bax/Bcl-2 ratio, and cytokines secretion. Wine le...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030222001667 |
| _version_ | 1811330900072333312 |
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| author | M.G. Ciliberti M. Francavilla M. Albenzio C. Inghese A. Santillo A. Sevi M. Caroprese |
| author_facet | M.G. Ciliberti M. Francavilla M. Albenzio C. Inghese A. Santillo A. Sevi M. Caroprese |
| author_sort | M.G. Ciliberti |
| collection | DOAJ |
| description | ABSTRACT: The objective of this study was to apply microwave-assisted extraction using green solvents starting from 3 different wine (white, rosé, and red) lees and to test their bio-response on sheep peripheral blood mononuclear cells proliferation, Bax/Bcl-2 ratio, and cytokines secretion. Wine lees (WL) of local organic farming from white wine, produced with Trebbiano cultivar, rosé and red wine, made with Nero di Troia cultivar, were collected. The WL were subjected to microwave-assisted extraction using 2 green solvents (water and ethanol) in 3 different combinations (water; water/ethanol 1:1 vol/vol; ethanol) with a dry matter-to-solvent ratio of 1:40 (wt/vol) at 4 temperature levels: 50, 100, 150, and 200°C. Sodium carbonate Na2CO3 (2 mmol/g of dry weight of lees) was used for increasing the polyphenol extraction yield. A total number of 6 extracts, 2 for each kind of WL investigated, according to their total phenolic content and in vitro antioxidant capacity, were selected to be tested on sheep peripheral blood mononuclear cells, as an animal model. All the WL extracts demonstrated a strong antiproliferative action. On the contrary, the cytokines' profile was mainly dependent on the different winemaking derived WL and the extraction solvent combination procedures. Red WL extract obtained by a combination of water/Na2CO3 and tested at 0.8 mg/mL, resulted in an increase of both IL-6 secretion and Bax/Bcl-2 ratio. Data from the present study demonstrated that WL extracts derived from different winemaking and solvent extraction could have a bimodal action on control of inflammatory mediated damage and highlighted the importance for further studies aimed at applying the biorefinery process on by-products to increase their economic value and exploit new derived bioactive compound. |
| first_indexed | 2024-04-13T16:10:50Z |
| format | Article |
| id | doaj.art-5c9ad17388c94a9eb6e9afa0ef822b2f |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-04-13T16:10:50Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-5c9ad17388c94a9eb6e9afa0ef822b2f2022-12-22T02:40:15ZengElsevierJournal of Dairy Science0022-03022022-05-01105543354353Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep modelM.G. Ciliberti0M. Francavilla1M. Albenzio2C. Inghese3A. Santillo4A. Sevi5M. Caroprese6Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy; Corresponding authorDepartment of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy; STAR Facility Centre, Department of Agriculture, Food, Natural Resources and Engineering (DAFNE), University of Foggia, Via Napoli 25, 71122 Foggia, ItalyDepartment of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, ItalyNutritionist, San Severo, 71016 Foggia, ItalyDepartment of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, ItalyDepartment of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, ItalyDepartment of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, ItalyABSTRACT: The objective of this study was to apply microwave-assisted extraction using green solvents starting from 3 different wine (white, rosé, and red) lees and to test their bio-response on sheep peripheral blood mononuclear cells proliferation, Bax/Bcl-2 ratio, and cytokines secretion. Wine lees (WL) of local organic farming from white wine, produced with Trebbiano cultivar, rosé and red wine, made with Nero di Troia cultivar, were collected. The WL were subjected to microwave-assisted extraction using 2 green solvents (water and ethanol) in 3 different combinations (water; water/ethanol 1:1 vol/vol; ethanol) with a dry matter-to-solvent ratio of 1:40 (wt/vol) at 4 temperature levels: 50, 100, 150, and 200°C. Sodium carbonate Na2CO3 (2 mmol/g of dry weight of lees) was used for increasing the polyphenol extraction yield. A total number of 6 extracts, 2 for each kind of WL investigated, according to their total phenolic content and in vitro antioxidant capacity, were selected to be tested on sheep peripheral blood mononuclear cells, as an animal model. All the WL extracts demonstrated a strong antiproliferative action. On the contrary, the cytokines' profile was mainly dependent on the different winemaking derived WL and the extraction solvent combination procedures. Red WL extract obtained by a combination of water/Na2CO3 and tested at 0.8 mg/mL, resulted in an increase of both IL-6 secretion and Bax/Bcl-2 ratio. Data from the present study demonstrated that WL extracts derived from different winemaking and solvent extraction could have a bimodal action on control of inflammatory mediated damage and highlighted the importance for further studies aimed at applying the biorefinery process on by-products to increase their economic value and exploit new derived bioactive compound.http://www.sciencedirect.com/science/article/pii/S0022030222001667wine leescircular economygreen solventsustainability |
| spellingShingle | M.G. Ciliberti M. Francavilla M. Albenzio C. Inghese A. Santillo A. Sevi M. Caroprese Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model Journal of Dairy Science wine lees circular economy green solvent sustainability |
| title | Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model |
| title_full | Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model |
| title_fullStr | Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model |
| title_full_unstemmed | Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model |
| title_short | Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model |
| title_sort | green extraction of bioactive compounds from wine lees and their bio responses on immune modulation using in vitro sheep model |
| topic | wine lees circular economy green solvent sustainability |
| url | http://www.sciencedirect.com/science/article/pii/S0022030222001667 |
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