An ultra-sensitive suboptimal protospacer adjacent motif enhanced rolling circle amplification assay based on CRISPR/Cas12a for detection of miR-183

An ultra-sensitive suboptimal protospacer adjacent motif enhanced rolling circle amplification assay based on CRISPR/Cas12a for detection of miR-183

IntroductionMicroRNAs (miRNAs) have been recognized as promising diagnostic biomarkers for Diabetic Retinopathy (DR) due to their notable upregulation in individuals with the condition. However, the development of highly sensitive miRNAs assays for the rapid diagnosis of DR in clinical settings rema...

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Bibliographic Details
Main Authors: Zhiquan Lu, Shijing Wang, Ping Li, Huasheng Yang, Sanyang Han, Shaochong Zhang, Lan Ma
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-09-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
diabetic retinopathy
CRISPR/Cas12a
microRNA
rolling circle amplification
fluorescence
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2024.1444908/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2024.1444908/full

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