Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro
This study aimed to determine the effect of capsicum oleoresin (CAP) on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro. The experimental design was arranged in a 2 × 2 × 3 factorial format together with two temperatures (normal: 39°C; hyperther...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005818/full |
| _version_ | 1798001341592764416 |
|---|---|
| author | Zhigao An Zhigao An Gan Luo Gan Luo Mohamed Abdelrahman Mohamed Abdelrahman Umair Riaz Umair Riaz Umair Riaz Shanshan Gao Shanshan Gao Zhiqiu Yao Zhiqiu Yao Tingzhu Ye Tingzhu Ye Haimiao Lv Haimiao Lv Jvnwei Zhao Jvnwei Zhao Changzhi Chen Liguo Yang Liguo Yang Liguo Yang |
| author_facet | Zhigao An Zhigao An Gan Luo Gan Luo Mohamed Abdelrahman Mohamed Abdelrahman Umair Riaz Umair Riaz Umair Riaz Shanshan Gao Shanshan Gao Zhiqiu Yao Zhiqiu Yao Tingzhu Ye Tingzhu Ye Haimiao Lv Haimiao Lv Jvnwei Zhao Jvnwei Zhao Changzhi Chen Liguo Yang Liguo Yang Liguo Yang |
| author_sort | Zhigao An |
| collection | DOAJ |
| description | This study aimed to determine the effect of capsicum oleoresin (CAP) on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro. The experimental design was arranged in a 2 × 2 × 3 factorial format together with two temperatures (normal: 39°C; hyperthermal: 42°C), two forage/concentrate ratios (30:70; 70:30), and two CAP concentrations in the incubation fluid at 20 and 200 mg/L with a control group. Regarding the fermentation characteristics, high temperature reduced short-chain fatty acids (SCFA) production except for molar percentages of butyrate while increasing acetate-to-propionate ratio and ammonia concentration. The diets increased total SCFA, propionate, and ammonia concentrations while decreasing acetate percentage and acetate-to-propionate ratio. CAP reduced acetate percentage and acetate-to-propionate ratio. Under hyperthermal condition, CAP could reduce acetate percentage and increase acetate-to-propionate ratio, lessening the negative effect of high heat on SCFA. Hyperthermal condition and diet altered the relative abundance of microbial abundance in cellulose-degrading bacteria. CAP showed little effect on the microbial abundance which only increased Butyrivibrio fibrisolvens. Thus, CAP could improve rumen fermentation under different conditions, with plasticity in response to the ramp of different temperature and dietary conditions, although hardly affecting rumen microbial abundance. |
| first_indexed | 2024-04-11T11:34:31Z |
| format | Article |
| id | doaj.art-5d2303b91af84418a24c23001b2d0bc4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T11:34:31Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5d2303b91af84418a24c23001b2d0bc42022-12-22T04:26:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.10058181005818Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitroZhigao An0Zhigao An1Gan Luo2Gan Luo3Mohamed Abdelrahman4Mohamed Abdelrahman5Umair Riaz6Umair Riaz7Umair Riaz8Shanshan Gao9Shanshan Gao10Zhiqiu Yao11Zhiqiu Yao12Tingzhu Ye13Tingzhu Ye14Haimiao Lv15Haimiao Lv16Jvnwei Zhao17Jvnwei Zhao18Changzhi Chen19Liguo Yang20Liguo Yang21Liguo Yang22Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaAnimal Production Department, Faculty of Agriculture, Assiut University, Asyut, EgyptKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaFaculty of Veterinary and Animal Sciences, Islamia University of Bahawalpur, Bahawalpur, PakistanKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaBright Farming Co. Ltd., Shanghai, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Joint Research Centre for Animal Genetics, Breeding and Reproduction (IJRCAGBR), Huazhong Agricultural University, Wuhan, ChinaHubei Province’s Engineering Research Center in Buffalo Breeding and Products, Wuhan, ChinaThis study aimed to determine the effect of capsicum oleoresin (CAP) on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro. The experimental design was arranged in a 2 × 2 × 3 factorial format together with two temperatures (normal: 39°C; hyperthermal: 42°C), two forage/concentrate ratios (30:70; 70:30), and two CAP concentrations in the incubation fluid at 20 and 200 mg/L with a control group. Regarding the fermentation characteristics, high temperature reduced short-chain fatty acids (SCFA) production except for molar percentages of butyrate while increasing acetate-to-propionate ratio and ammonia concentration. The diets increased total SCFA, propionate, and ammonia concentrations while decreasing acetate percentage and acetate-to-propionate ratio. CAP reduced acetate percentage and acetate-to-propionate ratio. Under hyperthermal condition, CAP could reduce acetate percentage and increase acetate-to-propionate ratio, lessening the negative effect of high heat on SCFA. Hyperthermal condition and diet altered the relative abundance of microbial abundance in cellulose-degrading bacteria. CAP showed little effect on the microbial abundance which only increased Butyrivibrio fibrisolvens. Thus, CAP could improve rumen fermentation under different conditions, with plasticity in response to the ramp of different temperature and dietary conditions, although hardly affecting rumen microbial abundance.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005818/fullcapsicum oleoresinhyperthermal stressdietary conditionsrumen fermentationmicrobial abundance |
| spellingShingle | Zhigao An Zhigao An Gan Luo Gan Luo Mohamed Abdelrahman Mohamed Abdelrahman Umair Riaz Umair Riaz Umair Riaz Shanshan Gao Shanshan Gao Zhiqiu Yao Zhiqiu Yao Tingzhu Ye Tingzhu Ye Haimiao Lv Haimiao Lv Jvnwei Zhao Jvnwei Zhao Changzhi Chen Liguo Yang Liguo Yang Liguo Yang Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro Frontiers in Microbiology capsicum oleoresin hyperthermal stress dietary conditions rumen fermentation microbial abundance |
| title | Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro |
| title_full | Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro |
| title_fullStr | Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro |
| title_full_unstemmed | Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro |
| title_short | Effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro |
| title_sort | effects of capsicum oleoresin supplementation on rumen fermentation and microbial abundance under different temperature and dietary conditions in vitro |
| topic | capsicum oleoresin hyperthermal stress dietary conditions rumen fermentation microbial abundance |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1005818/full |
| work_keys_str_mv | AT zhigaoan effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT zhigaoan effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT ganluo effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT ganluo effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT mohamedabdelrahman effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT mohamedabdelrahman effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT umairriaz effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT umairriaz effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT umairriaz effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT shanshangao effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT shanshangao effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT zhiqiuyao effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT zhiqiuyao effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT tingzhuye effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT tingzhuye effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT haimiaolv effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT haimiaolv effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT jvnweizhao effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT jvnweizhao effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT changzhichen effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT liguoyang effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT liguoyang effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro AT liguoyang effectsofcapsicumoleoresinsupplementationonrumenfermentationandmicrobialabundanceunderdifferenttemperatureanddietaryconditionsinvitro |