Profiling small RNAs in fecal immunochemical tests: is it possible?
Abstract Fecal microRNAs represent promising molecules with potential clinical interest as non-invasive diagnostic and prognostic biomarkers. Colorectal cancer (CRC) screening based on the fecal immunochemical test (FIT) is an effective tool for prevention of cancer development. However, due to the...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12943-023-01869-w |
| _version_ | 1827635331079340032 |
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| author | Einar Birkeland Giulio Ferrero Barbara Pardini Sinan U. Umu Sonia Tarallo Sara Bulfamante Geir Hoff Carlo Senore Trine B Rounge Alessio Naccarati |
| author_facet | Einar Birkeland Giulio Ferrero Barbara Pardini Sinan U. Umu Sonia Tarallo Sara Bulfamante Geir Hoff Carlo Senore Trine B Rounge Alessio Naccarati |
| author_sort | Einar Birkeland |
| collection | DOAJ |
| description | Abstract Fecal microRNAs represent promising molecules with potential clinical interest as non-invasive diagnostic and prognostic biomarkers. Colorectal cancer (CRC) screening based on the fecal immunochemical test (FIT) is an effective tool for prevention of cancer development. However, due to the poor sensitivity of FIT especially for premalignant lesions, there is a need for implementation of complementary tests. Improving the identification of individuals who would benefit from further investigation with colonoscopy using molecular analysis, such as miRNA profiling of FIT samples, would be ideal due to their widespread use. In the present study, we assessed the feasibility of applying small RNA sequencing to measure human miRNAs in FIT leftover buffer in samples from two European screening populations. We showed robust detection of miRNAs with profiles similar to those obtained from specimens sampled using the established protocol of RNA stabilizing buffers, or in long-term archived samples. Detected miRNAs exhibited differential abundances for CRC, advanced adenoma, and control samples that were consistent for FIT and RNA-stabilizing buffers. Interestingly, the sequencing data also allowed for concomitant evaluation of small RNA-based microbial profiles. We demonstrated that it is possible to explore the human miRNome in FIT leftover samples across populations and envision that the analysis of small RNA biomarkers can complement the FIT in large scale screening settings. |
| first_indexed | 2024-03-09T15:26:16Z |
| format | Article |
| id | doaj.art-5d377c62ad044a339f559ffcc1c5b51a |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-03-09T15:26:16Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-5d377c62ad044a339f559ffcc1c5b51a2023-11-26T12:31:33ZengBMCMolecular Cancer1476-45982023-10-012211710.1186/s12943-023-01869-wProfiling small RNAs in fecal immunochemical tests: is it possible?Einar Birkeland0Giulio Ferrero1Barbara Pardini2Sinan U. Umu3Sonia Tarallo4Sara Bulfamante5Geir Hoff6Carlo Senore7Trine B Rounge8Alessio Naccarati9Centre for Bioinformatics, Department of Informatics, University of OsloDepartment of Clinical and Biological Sciences, University of TurinItalian Institute for Genomic Medicine (IIGM)Department of Pathology, Institute of Clinical Medicine, University of OsloItalian Institute for Genomic Medicine (IIGM)Epidemiology and Screening Unit-CPO, University Hospital Città della Salute e della ScienzaSection for colorectal cancer screening, Cancer Registry of Norway, Oslo University HospitalEpidemiology and Screening Unit-CPO, University Hospital Città della Salute e della ScienzaDepartment of Research, Cancer Registry of NorwayItalian Institute for Genomic Medicine (IIGM)Abstract Fecal microRNAs represent promising molecules with potential clinical interest as non-invasive diagnostic and prognostic biomarkers. Colorectal cancer (CRC) screening based on the fecal immunochemical test (FIT) is an effective tool for prevention of cancer development. However, due to the poor sensitivity of FIT especially for premalignant lesions, there is a need for implementation of complementary tests. Improving the identification of individuals who would benefit from further investigation with colonoscopy using molecular analysis, such as miRNA profiling of FIT samples, would be ideal due to their widespread use. In the present study, we assessed the feasibility of applying small RNA sequencing to measure human miRNAs in FIT leftover buffer in samples from two European screening populations. We showed robust detection of miRNAs with profiles similar to those obtained from specimens sampled using the established protocol of RNA stabilizing buffers, or in long-term archived samples. Detected miRNAs exhibited differential abundances for CRC, advanced adenoma, and control samples that were consistent for FIT and RNA-stabilizing buffers. Interestingly, the sequencing data also allowed for concomitant evaluation of small RNA-based microbial profiles. We demonstrated that it is possible to explore the human miRNome in FIT leftover samples across populations and envision that the analysis of small RNA biomarkers can complement the FIT in large scale screening settings.https://doi.org/10.1186/s12943-023-01869-wSmall RNA sequencingFecal immunochemical test (FIT)Colorectal cancer screeningStool biomarkersMicrobiomemicroRNAs |
| spellingShingle | Einar Birkeland Giulio Ferrero Barbara Pardini Sinan U. Umu Sonia Tarallo Sara Bulfamante Geir Hoff Carlo Senore Trine B Rounge Alessio Naccarati Profiling small RNAs in fecal immunochemical tests: is it possible? Molecular Cancer Small RNA sequencing Fecal immunochemical test (FIT) Colorectal cancer screening Stool biomarkers Microbiome microRNAs |
| title | Profiling small RNAs in fecal immunochemical tests: is it possible? |
| title_full | Profiling small RNAs in fecal immunochemical tests: is it possible? |
| title_fullStr | Profiling small RNAs in fecal immunochemical tests: is it possible? |
| title_full_unstemmed | Profiling small RNAs in fecal immunochemical tests: is it possible? |
| title_short | Profiling small RNAs in fecal immunochemical tests: is it possible? |
| title_sort | profiling small rnas in fecal immunochemical tests is it possible |
| topic | Small RNA sequencing Fecal immunochemical test (FIT) Colorectal cancer screening Stool biomarkers Microbiome microRNAs |
| url | https://doi.org/10.1186/s12943-023-01869-w |
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