Continuous Percoll Gradient Centrifugation of Erythrocytes—Explanation of Cellular Bands and Compromised Age Separation

(1) Background: When red blood cells are centrifuged in a continuous Percoll-based density gradient, they form discrete bands. While this is a popular approach for red blood cell age separation, the mechanisms involved in banding were unknown. (2) Methods: Percoll centrifugations of red blood cells...

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Bibliographic Details
Main Authors: Felix Maurer, Thomas John, Asya Makhro, Anna Bogdanova, Giampaolo Minetti, Christian Wagner, Lars Kaestner
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Cells
Subjects:
Online Access:https://www.mdpi.com/2073-4409/11/8/1296