Single-Cell Sorting of HBsAg-Binding Memory B Cells from Human Peripheral Blood Mononuclear Cells and Antibody Cloning

Single-Cell Sorting of HBsAg-Binding Memory B Cells from Human Peripheral Blood Mononuclear Cells and Antibody Cloning

Summary: The isolation of human antibodies with naturally paired heavy and light chains is crucial for understanding the human antibody immune response. Here, we present a protocol for antibody cloning from the sorted single human memory B cells recognizing hepatitis B virus (HBV) S antigen (HBsAg)....

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Bibliographic Details
Main Authors: Yunjiao Zhou, Zhenmi Liu, Zijun Wang, Qianqian Zhang, Christian T. Mayer, Till Schoofs, Michel C. Nussenzweig, Ype P. de Jong, Qiao Wang
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301167

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http://www.sciencedirect.com/science/article/pii/S2666166720301167

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