RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathway
Summary: RAF/MEK/ERK pathway is frequently activated in tumor. Therefore, this study will investigate the function of RUVBL1 (RAF-binding protein) in tongue squamous cell carcinoma (TSCC). Bioinformatics was performed to identify differentially expressed mRNAs (DE-mRNAs) in TCGA-oral squamous cell c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224006552 |
| _version_ | 1797253905984258048 |
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| author | Xin-yu Zhang Yang Liu Qiong Rong Ming-yue Qi Hui Guo |
| author_facet | Xin-yu Zhang Yang Liu Qiong Rong Ming-yue Qi Hui Guo |
| author_sort | Xin-yu Zhang |
| collection | DOAJ |
| description | Summary: RAF/MEK/ERK pathway is frequently activated in tumor. Therefore, this study will investigate the function of RUVBL1 (RAF-binding protein) in tongue squamous cell carcinoma (TSCC). Bioinformatics was performed to identify differentially expressed mRNAs (DE-mRNAs) in TCGA-oral squamous cell carcinoma, GSE13601, and GSE34105 datasets. A total of 672 shared DE-mRNAs were identified in three datasets, and they are regulating metastasis and angiogenesis. Patients with RUVBL1 low expression had high overall survival. Overexpressing RUVBL1 enhanced the viability, wound healing percentage, invasion, sphere formation, angiogenesis, and resistance to cisplatin and 5-fluorouracil in CAL-27 and SCC-4 cells, and the opposite results were obtained by knocking down RUVBL1. Moreover, overexpression of RUVBL1 bolstered tumor growth in vivo. Strikingly, RUVBL1 diminished the phosphorylation of CRAF Ser259, which led to activation of the MEK/ERK pathway. In conclusion, RUVBL1 contributes to the malignant biological behavior of TSCC via activating the CRAF/MEK/ERK pathway. This provides molecular mechanisms and perspectives for targeted therapy of the CRAF/MEK/ERK pathway. |
| first_indexed | 2024-04-24T21:41:30Z |
| format | Article |
| id | doaj.art-5d85d5f88b494614a0282e933ec4cc4a |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-24T21:41:30Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-5d85d5f88b494614a0282e933ec4cc4a2024-03-21T05:37:17ZengElsevieriScience2589-00422024-04-01274109434RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathwayXin-yu Zhang0Yang Liu1Qiong Rong2Ming-yue Qi3Hui Guo4The First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province 650032, ChinaKunming University of Science and Technology, Kunming, Yunnan Province 650500, ChinaThe First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province 650032, ChinaKunming University of Science and Technology, Kunming, Yunnan Province 650500, ChinaThe First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan Province 650032, China; Corresponding authorSummary: RAF/MEK/ERK pathway is frequently activated in tumor. Therefore, this study will investigate the function of RUVBL1 (RAF-binding protein) in tongue squamous cell carcinoma (TSCC). Bioinformatics was performed to identify differentially expressed mRNAs (DE-mRNAs) in TCGA-oral squamous cell carcinoma, GSE13601, and GSE34105 datasets. A total of 672 shared DE-mRNAs were identified in three datasets, and they are regulating metastasis and angiogenesis. Patients with RUVBL1 low expression had high overall survival. Overexpressing RUVBL1 enhanced the viability, wound healing percentage, invasion, sphere formation, angiogenesis, and resistance to cisplatin and 5-fluorouracil in CAL-27 and SCC-4 cells, and the opposite results were obtained by knocking down RUVBL1. Moreover, overexpression of RUVBL1 bolstered tumor growth in vivo. Strikingly, RUVBL1 diminished the phosphorylation of CRAF Ser259, which led to activation of the MEK/ERK pathway. In conclusion, RUVBL1 contributes to the malignant biological behavior of TSCC via activating the CRAF/MEK/ERK pathway. This provides molecular mechanisms and perspectives for targeted therapy of the CRAF/MEK/ERK pathway.http://www.sciencedirect.com/science/article/pii/S2589004224006552Molecular biologyCell biologyCancer |
| spellingShingle | Xin-yu Zhang Yang Liu Qiong Rong Ming-yue Qi Hui Guo RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathway iScience Molecular biology Cell biology Cancer |
| title | RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathway |
| title_full | RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathway |
| title_fullStr | RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathway |
| title_full_unstemmed | RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathway |
| title_short | RUVBL1 accelerates tongue squamous cell carcinoma by mediating CRaf/MEK/ERK pathway |
| title_sort | ruvbl1 accelerates tongue squamous cell carcinoma by mediating craf mek erk pathway |
| topic | Molecular biology Cell biology Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224006552 |
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