Real-time PCR method for detection of short DNA using a deoxyuridine probe and application for detection of fomivirsen
This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase. The extended DNA was used for real-time PCR after the probe was removed by uracil DNA glyco...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2022-12-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0068 |