Protocol to use de-epithelialized porcine urinary bladder as a tissue scaffold for propagation of pancreatic cells

Protocol to use de-epithelialized porcine urinary bladder as a tissue scaffold for propagation of pancreatic cells

Summary: Ex vivo organ culture can be a useful alternative to in vivo models, which can be time-, labor-, and cost-intensive. Here we describe a step-by-step protocol to use de-epithelialized porcine urinary bladders as scaffolds in air-liquid interface in vitro culture systems for a variety of plur...

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Bibliographic Details
Main Authors: Michael Karl Melzer, Markus Breunig, Paul Lopatta, Meike Hohwieler, Sarah Merz, Anca Azoitei, Cagatay Günes, Christian Bolenz, Alexander Kleger
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell culture
Stem Cells
Cell Differentiation
Tissue Engineering
Material sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007493

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http://www.sciencedirect.com/science/article/pii/S2666166722007493

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