Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
Stripe rust, which is caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid &...
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MDPI AG
2023-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/12/2311 |
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| author | Biran Gong Lei Zhao Chunyan Zeng Wei Zhu Lili Xu Dandan Wu Yiran Cheng Yi Wang Jian Zeng Xing Fan Lina Sha Haiqin Zhang Guoyue Chen Yonghong Zhou Houyang Kang |
| author_facet | Biran Gong Lei Zhao Chunyan Zeng Wei Zhu Lili Xu Dandan Wu Yiran Cheng Yi Wang Jian Zeng Xing Fan Lina Sha Haiqin Zhang Guoyue Chen Yonghong Zhou Houyang Kang |
| author_sort | Biran Gong |
| collection | DOAJ |
| description | Stripe rust, which is caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i>Thinopyrum elongatum</i> (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i>Th. elongatum</i> 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i>Th. elongatum</i>, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i>Th. elongatum,</i> which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i>Th. elongatum</i> from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i>Th. elongatum</i>. |
| first_indexed | 2024-03-11T02:00:58Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T02:00:58Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-5dbd20ceaf2543888265f310acddb9fc2023-11-18T12:10:21ZengMDPI AGPlants2223-77472023-06-011212231110.3390/plants12122311Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult StageBiran Gong0Lei Zhao1Chunyan Zeng2Wei Zhu3Lili Xu4Dandan Wu5Yiran Cheng6Yi Wang7Jian Zeng8Xing Fan9Lina Sha10Haiqin Zhang11Guoyue Chen12Yonghong Zhou13Houyang Kang14State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaStripe rust, which is caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid <i>Thinopyrum elongatum</i> (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid <i>Th. elongatum</i> 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid <i>Th. elongatum</i>, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid <i>Th. elongatum,</i> which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish <i>Th. elongatum</i> from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid <i>Th. elongatum</i>.https://www.mdpi.com/2223-7747/12/12/2311chromosomal substitutionmolecular markersstripe rusttetraploid <i>Thinopyrum elongatum</i> |
| spellingShingle | Biran Gong Lei Zhao Chunyan Zeng Wei Zhu Lili Xu Dandan Wu Yiran Cheng Yi Wang Jian Zeng Xing Fan Lina Sha Haiqin Zhang Guoyue Chen Yonghong Zhou Houyang Kang Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage Plants chromosomal substitution molecular markers stripe rust tetraploid <i>Thinopyrum elongatum</i> |
| title | Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage |
| title_full | Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage |
| title_fullStr | Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage |
| title_full_unstemmed | Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage |
| title_short | Development and Characterization of a Novel Wheat–Tetraploid <i>Thinopyrum elongatum</i> 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage |
| title_sort | development and characterization of a novel wheat tetraploid i thinopyrum elongatum i 6e 6d disomic substitution line with stripe rust resistance at the adult stage |
| topic | chromosomal substitution molecular markers stripe rust tetraploid <i>Thinopyrum elongatum</i> |
| url | https://www.mdpi.com/2223-7747/12/12/2311 |
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