Arrayed CRISPRi and quantitative imaging describe the morphotypic landscape of essential mycobacterial genes

Arrayed CRISPRi and quantitative imaging describe the morphotypic landscape of essential mycobacterial genes

Mycobacterium tuberculosis possesses a large number of genes of unknown or predicted function, undermining fundamental understanding of pathogenicity and drug susceptibility. To address this challenge, we developed a high-throughput functional genomics approach combining inducible CRISPR-interferenc...

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Bibliographic Details
Main Authors: Timothy J de Wet, Kristy R Winkler, Musa Mhlanga, Valerie Mizrahi, Digby F Warner
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-11-01
Series:eLife
Subjects:
Mycobacterium tuberculosis
Mycobacterium smegmatis
CRISPRi
phenoprint
UMAP
functional genomics
Online Access:https://elifesciences.org/articles/60083

Internet

https://elifesciences.org/articles/60083

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