Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophages
BackgroundSparassis latifolia (S. latifolia) is a precious edible fungus with multiple biological activities. To date, no study has been investigated the underlying molecular mechanism of immunoregulation caused by the neutral polysaccharide of S. latifolia.Materials and methodsTo investigate immuno...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.994971/full |
| _version_ | 1828231054102626304 |
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| author | Zening Qiao Yue Zhao Menghao Wang Jinling Cao Mingchang Chang Shaojun Yun Yanfen Cheng Feier Cheng Cuiping Feng |
| author_facet | Zening Qiao Yue Zhao Menghao Wang Jinling Cao Mingchang Chang Shaojun Yun Yanfen Cheng Feier Cheng Cuiping Feng |
| author_sort | Zening Qiao |
| collection | DOAJ |
| description | BackgroundSparassis latifolia (S. latifolia) is a precious edible fungus with multiple biological activities. To date, no study has been investigated the underlying molecular mechanism of immunoregulation caused by the neutral polysaccharide of S. latifolia.Materials and methodsTo investigate immunomodulatory mechanism of S. latifolia neutral polysaccharide (SLNP), SLNP was obtained from S. latifolia and its structure, immune receptors and regulation mechanism were studied.ResultsS. latifolia neutral polysaccharide consisted of arabinose, galactose, glucose, xylose, and mannose with a molar ratio of 6:12:63:10:5. SLNP was a pyran polysaccharide with a relative molecular weight of 3.2 × 105 Da. SLNP promoted the proliferation of RAW264.7, which further induced the secretions of nitric oxide, TNF-α, IL-6, and IFN-β, and upregulated the immune receptor TLR4 expression. Moreover, SLNP increased remarkably the levels of TRAF6, IRF3, JNK, ERK, p38, and p38 mRNA and protein mediated by TLR4.ConclusionS. latifolia neutral polysaccharide regulated the immune function of RAW264.7 through MyD88-dependent and -independent signaling pathways mediated by TLR4 receptor, which suggests that SLNP is a new immunomodulator. |
| first_indexed | 2024-04-12T18:58:44Z |
| format | Article |
| id | doaj.art-5de2d5fcf929467fba3897a705a533d9 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T18:58:44Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-5de2d5fcf929467fba3897a705a533d92022-12-22T03:20:15ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.994971994971Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophagesZening QiaoYue ZhaoMenghao WangJinling CaoMingchang ChangShaojun YunYanfen ChengFeier ChengCuiping FengBackgroundSparassis latifolia (S. latifolia) is a precious edible fungus with multiple biological activities. To date, no study has been investigated the underlying molecular mechanism of immunoregulation caused by the neutral polysaccharide of S. latifolia.Materials and methodsTo investigate immunomodulatory mechanism of S. latifolia neutral polysaccharide (SLNP), SLNP was obtained from S. latifolia and its structure, immune receptors and regulation mechanism were studied.ResultsS. latifolia neutral polysaccharide consisted of arabinose, galactose, glucose, xylose, and mannose with a molar ratio of 6:12:63:10:5. SLNP was a pyran polysaccharide with a relative molecular weight of 3.2 × 105 Da. SLNP promoted the proliferation of RAW264.7, which further induced the secretions of nitric oxide, TNF-α, IL-6, and IFN-β, and upregulated the immune receptor TLR4 expression. Moreover, SLNP increased remarkably the levels of TRAF6, IRF3, JNK, ERK, p38, and p38 mRNA and protein mediated by TLR4.ConclusionS. latifolia neutral polysaccharide regulated the immune function of RAW264.7 through MyD88-dependent and -independent signaling pathways mediated by TLR4 receptor, which suggests that SLNP is a new immunomodulator.https://www.frontiersin.org/articles/10.3389/fnut.2022.994971/fullSparassis latifolianeutral polysaccharidemacrophage RAW264.7immune activitytoll-like receptor-4MyD88 signaling pathway |
| spellingShingle | Zening Qiao Yue Zhao Menghao Wang Jinling Cao Mingchang Chang Shaojun Yun Yanfen Cheng Feier Cheng Cuiping Feng Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophages Frontiers in Nutrition Sparassis latifolia neutral polysaccharide macrophage RAW264.7 immune activity toll-like receptor-4 MyD88 signaling pathway |
| title | Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophages |
| title_full | Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophages |
| title_fullStr | Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophages |
| title_full_unstemmed | Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophages |
| title_short | Effects of Sparassis latifolia neutral polysaccharide on immune activity via TLR4-mediated MyD88-dependent and independent signaling pathways in RAW264.7 macrophages |
| title_sort | effects of sparassis latifolia neutral polysaccharide on immune activity via tlr4 mediated myd88 dependent and independent signaling pathways in raw264 7 macrophages |
| topic | Sparassis latifolia neutral polysaccharide macrophage RAW264.7 immune activity toll-like receptor-4 MyD88 signaling pathway |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.994971/full |
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