Protocol for optimizing production and quality control of infective EcoHIV virions

Protocol for optimizing production and quality control of infective EcoHIV virions

Summary: EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps fo...

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Bibliographic Details
Main Authors: Hammodah R. Alfar, Daphne N. Pariser, Harry Chanzu, Smita Joshi, Daniëlle M. Coenen, Joshua Lykins, Kanakanagavalli Shravani Prakhya, Mary Jane Potash, Wei Chao, Jennifer Kelschenbach, David J. Volsky, Kelly Metcalf-Pate, Sidney W. Whiteheart
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Cell Culture
Cell-based Assays
Health Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003350

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http://www.sciencedirect.com/science/article/pii/S2666166723003350

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