A Python script to design primers for overlap extension PCR to ligate two DNA fragments
Ligating two or more DNA fragments is a regular operation for the subcloning and the engineering of vectors. The overlap extension PCR serves as a straightforward method to solve this issue. However, it takes a relatively long time to design the appropriate overlapping primers and the primers for th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2022-10-01
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| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/14283.pdf |