Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with p...
Main Authors: | , , , , , |
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Format: | Article |
Jezik: | English |
Izdano: |
Instituto de Tecnologia do Paraná (Tecpar)
2006-07-01
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Serija: | Brazilian Archives of Biology and Technology |
Teme: | |
Online dostop: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500003 |