Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in Turkey
The olive (Olea europaea L.) is one of the most important plants grown in many Mediterranean countries that has a high economic value. Olives, which are specific to each region, have different bioactive components. In this study, we investigated the phenolic/flavonoid contents, as well as antioxidan...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kemerovo State University
2023-03-01
|
| Series: | Foods and Raw Materials |
| Subjects: | |
| Online Access: | https://jfrm.ru/en/issues/20672/21264/ |
| _version_ | 1797850900628242432 |
|---|---|
| author | Kadir Batçıoğlu F. Zehra Küçükbay Mehmet Abdullah Alagöz Selami Günal Yakup Yilmaztekin |
| author_facet | Kadir Batçıoğlu F. Zehra Küçükbay Mehmet Abdullah Alagöz Selami Günal Yakup Yilmaztekin |
| author_sort | Kadir Batçıoğlu |
| collection | DOAJ |
| description | The olive (Olea europaea L.) is one of the most important plants grown in many Mediterranean countries that has a high economic value. Olives, which are specific to each region, have different bioactive components. In this study, we investigated the phenolic/flavonoid contents, as well as antioxidant, antimicrobial, and antithrombotic activities of the fruit, leaf, and seed extracts obtained from the Halhalı olive grown in Arsuz district of Hatay, Turkey.
Antioxidant activities of the phenolic compounds found in the olive fruit, seed, and leaf extracts were determined by employing established in vitro systems. Total phenolics were determined as gallic acid equivalents, while total flavonoids were determined as quercetin equivalents. Also, we evaluated a possible interaction between oleuropein and aggregation-related glycoproteins of the platelet surface via docking studies.
The extracts showed effective antioxidant activity. The seed extract had the highest phenolic content of 317.24 μg GAE, while the fruit extract had the highest flavonoid content of 4.43 μg. The highest potential for metal chelating activity was found in the leaf extract, with an IC50 value of 13.33 mg/mL. Also, the leaf extract showed higher levels of antioxidant, antithrombotic, and antimicrobial activity, compared to the fruit and seed extracts. The docking scores of oleuropein against the target molecules GPVI, α2β1, and GPIbα were calculated as –3.798, –4.315, and –6.464 kcal/mol, respectively.
The olive fruit, leaf, and seed extracts used as experimental material in our study have remarkable antioxidant, antimicrobial, and antithrombotic potential. |
| first_indexed | 2024-04-09T19:09:08Z |
| format | Article |
| id | doaj.art-5e36b6f6819c4f61808b46002cc6c0ca |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-04-09T19:09:08Z |
| publishDate | 2023-03-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-5e36b6f6819c4f61808b46002cc6c0ca2023-04-07T06:48:05ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992023-03-01111849310.21603/2308-4057-2023-1-557Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in TurkeyKadir Batçıoğlu0https://orcid.org/0000-0001-6663-2287F. Zehra Küçükbay1https://orcid.org/0000-0001-7784-4138Mehmet Abdullah Alagöz2https://orcid.org/0000-0001-5190-7196Selami Günal 3https://orcid.org/0000-0002-4752-5176Yakup Yilmaztekin4https://orcid.org/0000-0002-8676-4487Inönü University, Battalgazi, TurkeyInönü University, Battalgazi, TurkeyInönü University, Battalgazi, TurkeyInönü University, Battalgazi, TurkeyInönü University, Battalgazi, TurkeyThe olive (Olea europaea L.) is one of the most important plants grown in many Mediterranean countries that has a high economic value. Olives, which are specific to each region, have different bioactive components. In this study, we investigated the phenolic/flavonoid contents, as well as antioxidant, antimicrobial, and antithrombotic activities of the fruit, leaf, and seed extracts obtained from the Halhalı olive grown in Arsuz district of Hatay, Turkey. Antioxidant activities of the phenolic compounds found in the olive fruit, seed, and leaf extracts were determined by employing established in vitro systems. Total phenolics were determined as gallic acid equivalents, while total flavonoids were determined as quercetin equivalents. Also, we evaluated a possible interaction between oleuropein and aggregation-related glycoproteins of the platelet surface via docking studies. The extracts showed effective antioxidant activity. The seed extract had the highest phenolic content of 317.24 μg GAE, while the fruit extract had the highest flavonoid content of 4.43 μg. The highest potential for metal chelating activity was found in the leaf extract, with an IC50 value of 13.33 mg/mL. Also, the leaf extract showed higher levels of antioxidant, antithrombotic, and antimicrobial activity, compared to the fruit and seed extracts. The docking scores of oleuropein against the target molecules GPVI, α2β1, and GPIbα were calculated as –3.798, –4.315, and –6.464 kcal/mol, respectively. The olive fruit, leaf, and seed extracts used as experimental material in our study have remarkable antioxidant, antimicrobial, and antithrombotic potential.https://jfrm.ru/en/issues/20672/21264/olea europaea l.antioxidantantithrombotic activityantimicrobial activitymolecular docking |
| spellingShingle | Kadir Batçıoğlu F. Zehra Küçükbay Mehmet Abdullah Alagöz Selami Günal Yakup Yilmaztekin Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in Turkey Foods and Raw Materials olea europaea l. antioxidant antithrombotic activity antimicrobial activity molecular docking |
| title | Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in Turkey |
| title_full | Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in Turkey |
| title_fullStr | Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in Turkey |
| title_full_unstemmed | Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in Turkey |
| title_short | Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhalı olive (Olea europaea L.) native to the Hatay region in Turkey |
| title_sort | antioxidant and antithrombotic properties of fruit leaf and seed extracts of the halhali olive olea europaea l native to the hatay region in turkey |
| topic | olea europaea l. antioxidant antithrombotic activity antimicrobial activity molecular docking |
| url | https://jfrm.ru/en/issues/20672/21264/ |
| work_keys_str_mv | AT kadirbatcıoglu antioxidantandantithromboticpropertiesoffruitleafandseedextractsofthehalhalıoliveoleaeuropaealnativetothehatayregioninturkey AT fzehrakucukbay antioxidantandantithromboticpropertiesoffruitleafandseedextractsofthehalhalıoliveoleaeuropaealnativetothehatayregioninturkey AT mehmetabdullahalagoz antioxidantandantithromboticpropertiesoffruitleafandseedextractsofthehalhalıoliveoleaeuropaealnativetothehatayregioninturkey AT selamigunal antioxidantandantithromboticpropertiesoffruitleafandseedextractsofthehalhalıoliveoleaeuropaealnativetothehatayregioninturkey AT yakupyilmaztekin antioxidantandantithromboticpropertiesoffruitleafandseedextractsofthehalhalıoliveoleaeuropaealnativetothehatayregioninturkey |