Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass Spectrometry
To provide a scientific basis for the follow-up resource utilization and food development of Jinzhai hybrid G. elata, the ultra-high performance liquid chromatography coupled with mass spectrometry was utilized to accurately determine the contents of amino acids and nucleosides in Jinzhai Hongwu hyb...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030299 |
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| author | Guangzheng WANG Qingqing GONG Nianjun YU Hanbo GAO Lihua XING Fangping DU |
| author_facet | Guangzheng WANG Qingqing GONG Nianjun YU Hanbo GAO Lihua XING Fangping DU |
| author_sort | Guangzheng WANG |
| collection | DOAJ |
| description | To provide a scientific basis for the follow-up resource utilization and food development of Jinzhai hybrid G. elata, the ultra-high performance liquid chromatography coupled with mass spectrometry was utilized to accurately determine the contents of amino acids and nucleosides in Jinzhai Hongwu hybrid Gastrodia elata Bl. and its parents Yunnan G. elata Bl. f. glauca S. Chow and Jinzhai G. elata Bl. f. elata. Statistical methods were also applied to clarify and comprehensively evaluate the composition and content differences in amino acids and nucleosides among the three varieties of G. elata. C18 column (2.1 mm×100 mm, 1.8 μm) was used with 0.2% formic acid water (A)-acetonitrile (B) as the mobile phase at a flow rate of 0.2 mL/min. The column temperature was 30 ℃ with gradient elution. Moreover, electrospray ion source was measured in multiple reaction monitoring (MRM) mode, which was set as spray voltage at 5500 V; ionization temperature at 550 ℃; ion source gas pressure (Gas) at 241.3 kPa, atomizing gas (GS1) at 379.2 kPa, and auxiliary gas (GS2) at 379.2 kPa. The results revealed that there was a good linear relationship in the range of 0~144.58 μg/mL of the 31 components. The coefficient of determination R2 was greater than 0.999, and the RSD of the precision, stability, and repeatability of the peak area was not greater than 3.0%. The average recovery rate was between 92.69% and 99.5%, while recovery rate RSD ranged from 1.3% to 2.9%. Based on all above, G. elata was rich in amino acids and nucleotides, of which glutamic acid and aspartic acid were the main components. The contents of total amino acids and nucleosides in hybrid G. elata were higher than those of its parents G. elata. And there was a significant difference in the amino acids contents of different varieties (P<0.05). In the evaluation results of PCA and TOPSIS methods, the qualities of S1 and S2 hybrid G. elata were the best. This paper established a new method for evaluating the quality of G. elata and provided a scientific basis for the development and exploitation of Jinzhai hybrid varieties. |
| first_indexed | 2024-04-10T22:28:31Z |
| format | Article |
| id | doaj.art-5e443b701c434f168fdcde89032c1c1d |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-10T22:28:31Z |
| publishDate | 2023-02-01 |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-5e443b701c434f168fdcde89032c1c1d2023-01-17T08:07:44ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-02-0144326927810.13386/j.issn1002-0306.20220302992022030299-3Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass SpectrometryGuangzheng WANG0Qingqing GONG1Nianjun YU2Hanbo GAO3Lihua XING4Fangping DU5School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, ChinaSchool of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, ChinaSchool of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, ChinaSchool of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, ChinaSchool of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, ChinaJinzhai County Medicine Mushroom Planting Professional Cooperative, Liuan 237000, ChinaTo provide a scientific basis for the follow-up resource utilization and food development of Jinzhai hybrid G. elata, the ultra-high performance liquid chromatography coupled with mass spectrometry was utilized to accurately determine the contents of amino acids and nucleosides in Jinzhai Hongwu hybrid Gastrodia elata Bl. and its parents Yunnan G. elata Bl. f. glauca S. Chow and Jinzhai G. elata Bl. f. elata. Statistical methods were also applied to clarify and comprehensively evaluate the composition and content differences in amino acids and nucleosides among the three varieties of G. elata. C18 column (2.1 mm×100 mm, 1.8 μm) was used with 0.2% formic acid water (A)-acetonitrile (B) as the mobile phase at a flow rate of 0.2 mL/min. The column temperature was 30 ℃ with gradient elution. Moreover, electrospray ion source was measured in multiple reaction monitoring (MRM) mode, which was set as spray voltage at 5500 V; ionization temperature at 550 ℃; ion source gas pressure (Gas) at 241.3 kPa, atomizing gas (GS1) at 379.2 kPa, and auxiliary gas (GS2) at 379.2 kPa. The results revealed that there was a good linear relationship in the range of 0~144.58 μg/mL of the 31 components. The coefficient of determination R2 was greater than 0.999, and the RSD of the precision, stability, and repeatability of the peak area was not greater than 3.0%. The average recovery rate was between 92.69% and 99.5%, while recovery rate RSD ranged from 1.3% to 2.9%. Based on all above, G. elata was rich in amino acids and nucleotides, of which glutamic acid and aspartic acid were the main components. The contents of total amino acids and nucleosides in hybrid G. elata were higher than those of its parents G. elata. And there was a significant difference in the amino acids contents of different varieties (P<0.05). In the evaluation results of PCA and TOPSIS methods, the qualities of S1 and S2 hybrid G. elata were the best. This paper established a new method for evaluating the quality of G. elata and provided a scientific basis for the development and exploitation of Jinzhai hybrid varieties.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030299gastrodia elataliquid chromatography-tandem mass spectrometryamino acidnucleosideprincipal component analysistopsishomology of medicine and foodquality evaluation |
| spellingShingle | Guangzheng WANG Qingqing GONG Nianjun YU Hanbo GAO Lihua XING Fangping DU Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass Spectrometry Shipin gongye ke-ji gastrodia elata liquid chromatography-tandem mass spectrometry amino acid nucleoside principal component analysis topsis homology of medicine and food quality evaluation |
| title | Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Determination of Amino Acids and Nucleosides in Medicinal and Edible Hybrid Gastrodia elata and Its Parent Varieties Based on Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | determination of amino acids and nucleosides in medicinal and edible hybrid gastrodia elata and its parent varieties based on liquid chromatography tandem mass spectrometry |
| topic | gastrodia elata liquid chromatography-tandem mass spectrometry amino acid nucleoside principal component analysis topsis homology of medicine and food quality evaluation |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030299 |
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