Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus
Surface functionalization of nanoparticles has shown potential in enhancing the efficacy of antibiotic-loaded nanosystems against drug-resistant bacteria. The objective of this study was to synthesize and characterize an acid-cleavable pH-responsive polymer from methoxy polyethylene glycol and oleyl...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2024-01-01
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| Series: | Nano Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2632-959X/ad1d02 |
| _version_ | 1827376222201446400 |
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| author | Nawras Osman Calvin A Omolo Mohammed A Gafar Nikita Devnarain Sanjeev Rambharose Usri H Ibrahim Victoria O Fasiku Thirumala Govender |
| author_facet | Nawras Osman Calvin A Omolo Mohammed A Gafar Nikita Devnarain Sanjeev Rambharose Usri H Ibrahim Victoria O Fasiku Thirumala Govender |
| author_sort | Nawras Osman |
| collection | DOAJ |
| description | Surface functionalization of nanoparticles has shown potential in enhancing the efficacy of antibiotic-loaded nanosystems against drug-resistant bacteria. The objective of this study was to synthesize and characterize an acid-cleavable pH-responsive polymer from methoxy polyethylene glycol and oleylamine (mPEG-OA) to surface modify vancomycin (VCM)-loaded niosomes and to evaluate their antibacterial and anti-biofilm effectiveness against methicillin-resistant Staphylococcus aureus (MRSA). The novel mPEG-OA-coated niosomes were biocompatible, hemocompatible with size, polydispersity index, and zeta potential of 169.2 ± 1.6 nm, 0.21 ± 0.01 and −0.82 ± 0.22 mV, respectively. Under acidic conditions, mPEG-OA-coated niosomes exhibited a pH-responsive and sustained VCM release profile and in vitro antibacterial activity than non-coated niosomes and bare VCM. mPEG-OA-coated niosomes showed a significant reduction in biofilm formation at pH 6 compared to pH 7.4 ( p = 0,0119). The in vivo efficacy of mPEG-OA-coated niosomes in the BALB/c mice skin infection model showed a 9.9-fold reduction in MRSA load compared to bare VCM. Histomorphologically, the mPEG-OA-coated niosomes group displayed the lowest bacterial load, tissue swelling, and inflammation. The results of this study demonstrate the potential of novel pH-responsive mPEG-OA-derived polymer coating to enhance bacterial killing kinetics, and antibacterial and anti-biofilm efficacies over conventional antibiotic and non-functionalized nano delivery systems. |
| first_indexed | 2024-03-08T12:06:38Z |
| format | Article |
| id | doaj.art-5e56b9a437da475ab7ce3525a39e6d6f |
| institution | Directory Open Access Journal |
| issn | 2632-959X |
| language | English |
| last_indexed | 2024-03-08T12:06:38Z |
| publishDate | 2024-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Nano Express |
| spelling | doaj.art-5e56b9a437da475ab7ce3525a39e6d6f2024-01-23T07:17:52ZengIOP PublishingNano Express2632-959X2024-01-015101500810.1088/2632-959X/ad1d02Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureusNawras Osman0Calvin A Omolo1https://orcid.org/0000-0002-4421-3783Mohammed A Gafar2Nikita Devnarain3Sanjeev Rambharose4Usri H Ibrahim5Victoria O Fasiku6Thirumala Govender7Discipline of Pharmaceutical Sciences, College of Health Sciences, University of KwaZulu-Natal , Private Bag, Durban X54001, Durban, 4000, South Africa; Department of Pharmaceutics, Faculty of Pharmacy, University of Gezira , Wad Medani, SudanDiscipline of Pharmaceutical Sciences, College of Health Sciences, University of KwaZulu-Natal , Private Bag, Durban X54001, Durban, 4000, South Africa; Department of Pharmaceutics, School of Pharmacy and Health Sciences, United States International University-Africa , P. O. Box 14634-00800, Nairobi, KenyaDiscipline of Pharmaceutical Sciences, College of Health Sciences, University of KwaZulu-Natal , Private Bag, Durban X54001, Durban, 4000, South AfricaDiscipline of Pharmaceutical Sciences, College of Health Sciences, University of KwaZulu-Natal , Private Bag, Durban X54001, Durban, 4000, South AfricaDepartment of Physiological Sciences, Faculty of Science, Stellenbosch University , Private Bag X1 Matieland, Stellenbosch 7602, South AfricaDiscipline of Pharmaceutical Sciences, College of Health Sciences, University of KwaZulu-Natal , Private Bag, Durban X54001, Durban, 4000, South AfricaDiscipline of Pharmaceutical Sciences, College of Health Sciences, University of KwaZulu-Natal , Private Bag, Durban X54001, Durban, 4000, South AfricaDiscipline of Pharmaceutical Sciences, College of Health Sciences, University of KwaZulu-Natal , Private Bag, Durban X54001, Durban, 4000, South AfricaSurface functionalization of nanoparticles has shown potential in enhancing the efficacy of antibiotic-loaded nanosystems against drug-resistant bacteria. The objective of this study was to synthesize and characterize an acid-cleavable pH-responsive polymer from methoxy polyethylene glycol and oleylamine (mPEG-OA) to surface modify vancomycin (VCM)-loaded niosomes and to evaluate their antibacterial and anti-biofilm effectiveness against methicillin-resistant Staphylococcus aureus (MRSA). The novel mPEG-OA-coated niosomes were biocompatible, hemocompatible with size, polydispersity index, and zeta potential of 169.2 ± 1.6 nm, 0.21 ± 0.01 and −0.82 ± 0.22 mV, respectively. Under acidic conditions, mPEG-OA-coated niosomes exhibited a pH-responsive and sustained VCM release profile and in vitro antibacterial activity than non-coated niosomes and bare VCM. mPEG-OA-coated niosomes showed a significant reduction in biofilm formation at pH 6 compared to pH 7.4 ( p = 0,0119). The in vivo efficacy of mPEG-OA-coated niosomes in the BALB/c mice skin infection model showed a 9.9-fold reduction in MRSA load compared to bare VCM. Histomorphologically, the mPEG-OA-coated niosomes group displayed the lowest bacterial load, tissue swelling, and inflammation. The results of this study demonstrate the potential of novel pH-responsive mPEG-OA-derived polymer coating to enhance bacterial killing kinetics, and antibacterial and anti-biofilm efficacies over conventional antibiotic and non-functionalized nano delivery systems.https://doi.org/10.1088/2632-959X/ad1d02PEGylated niosomesoleylaminemethicillin-resistant staphylococcus aureuspH-responsivesurface modified nanoparticlesvancomycin |
| spellingShingle | Nawras Osman Calvin A Omolo Mohammed A Gafar Nikita Devnarain Sanjeev Rambharose Usri H Ibrahim Victoria O Fasiku Thirumala Govender Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus Nano Express PEGylated niosomes oleylamine methicillin-resistant staphylococcus aureus pH-responsive surface modified nanoparticles vancomycin |
| title | Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus |
| title_full | Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus |
| title_fullStr | Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus |
| title_short | Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus |
| title_sort | niosomes modified with a novel ph responsive coating mpeg oa enhance the antibacterial and anti biofilm activity of vancomycin against methicillin resistant staphylococcus aureus |
| topic | PEGylated niosomes oleylamine methicillin-resistant staphylococcus aureus pH-responsive surface modified nanoparticles vancomycin |
| url | https://doi.org/10.1088/2632-959X/ad1d02 |
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