Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling
Free radical generation and oxidative stress push forward an immense influence on the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. <i>Maclura tricuspidata</i> fruit (MT) contains many biologically active substances, including compounds w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/13/6946 |
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| author | Weishun Tian Suyoung Heo Dae-Woon Kim In-Shik Kim Dongchoon Ahn Hyun-Jin Tae Myung-Kon Kim Byung-Yong Park |
| author_facet | Weishun Tian Suyoung Heo Dae-Woon Kim In-Shik Kim Dongchoon Ahn Hyun-Jin Tae Myung-Kon Kim Byung-Yong Park |
| author_sort | Weishun Tian |
| collection | DOAJ |
| description | Free radical generation and oxidative stress push forward an immense influence on the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. <i>Maclura tricuspidata</i> fruit (MT) contains many biologically active substances, including compounds with antioxidant properties. The current study aimed to investigate the neuroprotective effects of MT fruit on hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced neurotoxicity in SH-SY5Y cells. SH-SY5Y cells were pretreated with MT, and cell damage was induced by H<sub>2</sub>O<sub>2</sub>. First, the chemical composition and free radical scavenging properties of MT were analyzed. MT attenuated oxidative stress-induced damage in cells based on the assessment of cell viability. The H<sub>2</sub>O<sub>2</sub>-induced toxicity caused by ROS production and lactate dehydrogenase (LDH) release was ameliorated by MT pretreatment. MT also promoted an increase in the expression of genes encoding the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). MT pretreatment was associated with an increase in the expression of neuronal genes downregulated by H<sub>2</sub>O<sub>2</sub>. Mechanistically, MT dramatically suppressed H<sub>2</sub>O<sub>2</sub>-induced Bcl-2 downregulation, Bax upregulation, apoptotic factor caspase-3 activation, Mitogen-activated protein kinase (MAPK) (JNK, ERK, and p38), and Nuclear factor-κB (NF-κB) activation, thereby preventing H<sub>2</sub>O<sub>2</sub>-induced neurotoxicity. These results indicate that MT has protective effects against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in SH-SY5Y cells and can be used to prevent and protect against neurodegeneration. |
| first_indexed | 2024-03-09T04:47:50Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:47:50Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5e90ead6bd634d03a52ac8ffc72fef6e2023-12-03T13:13:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213694610.3390/ijms22136946Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB SignalingWeishun Tian0Suyoung Heo1Dae-Woon Kim2In-Shik Kim3Dongchoon Ahn4Hyun-Jin Tae5Myung-Kon Kim6Byung-Yong Park7Bio-Safety Research Institute and College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, KoreaBio-Safety Research Institute and College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, KoreaDepartment of Food Science and Technology, Jeonbuk National University, Jeonju 54896, KoreaBio-Safety Research Institute and College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, KoreaBio-Safety Research Institute and College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, KoreaBio-Safety Research Institute and College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, KoreaDepartment of Food Science and Technology, Jeonbuk National University, Jeonju 54896, KoreaBio-Safety Research Institute and College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, KoreaFree radical generation and oxidative stress push forward an immense influence on the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. <i>Maclura tricuspidata</i> fruit (MT) contains many biologically active substances, including compounds with antioxidant properties. The current study aimed to investigate the neuroprotective effects of MT fruit on hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced neurotoxicity in SH-SY5Y cells. SH-SY5Y cells were pretreated with MT, and cell damage was induced by H<sub>2</sub>O<sub>2</sub>. First, the chemical composition and free radical scavenging properties of MT were analyzed. MT attenuated oxidative stress-induced damage in cells based on the assessment of cell viability. The H<sub>2</sub>O<sub>2</sub>-induced toxicity caused by ROS production and lactate dehydrogenase (LDH) release was ameliorated by MT pretreatment. MT also promoted an increase in the expression of genes encoding the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). MT pretreatment was associated with an increase in the expression of neuronal genes downregulated by H<sub>2</sub>O<sub>2</sub>. Mechanistically, MT dramatically suppressed H<sub>2</sub>O<sub>2</sub>-induced Bcl-2 downregulation, Bax upregulation, apoptotic factor caspase-3 activation, Mitogen-activated protein kinase (MAPK) (JNK, ERK, and p38), and Nuclear factor-κB (NF-κB) activation, thereby preventing H<sub>2</sub>O<sub>2</sub>-induced neurotoxicity. These results indicate that MT has protective effects against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in SH-SY5Y cells and can be used to prevent and protect against neurodegeneration.https://www.mdpi.com/1422-0067/22/13/6946<i>Maclura tricuspidata</i> (MT) fruitneuroprotectionhydrogen peroxideoxidative damageMAPKNF-κB |
| spellingShingle | Weishun Tian Suyoung Heo Dae-Woon Kim In-Shik Kim Dongchoon Ahn Hyun-Jin Tae Myung-Kon Kim Byung-Yong Park Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling International Journal of Molecular Sciences <i>Maclura tricuspidata</i> (MT) fruit neuroprotection hydrogen peroxide oxidative damage MAPK NF-κB |
| title | Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling |
| title_full | Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling |
| title_fullStr | Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling |
| title_full_unstemmed | Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling |
| title_short | Ethanol Extract of <i>Maclura tricuspidata</i> Fruit Protects SH-SY5Y Neuroblastoma Cells against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling |
| title_sort | ethanol extract of i maclura tricuspidata i fruit protects sh sy5y neuroblastoma cells against h sub 2 sub o sub 2 sub induced oxidative damage via inhibiting mapk and nf κb signaling |
| topic | <i>Maclura tricuspidata</i> (MT) fruit neuroprotection hydrogen peroxide oxidative damage MAPK NF-κB |
| url | https://www.mdpi.com/1422-0067/22/13/6946 |
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