Structural basis for lipid and copper regulation of the ABC transporter MsbA
The bacterial ABC transporter MsbA is essential for lipopolysaccharide biogenesis. Here, the authors apply native mass spectrometry, X-ray crystallography, cryo-EM and biochemical approaches to characterize the structural basis and functional roles of MsbA binding to copper and specific lipids.
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34905-2 |
| _version_ | 1828119797547663360 |
|---|---|
| author | Jixing Lyu Chang Liu Tianqi Zhang Samantha Schrecke Nicklaus P. Elam Charles Packianathan Georg K. A. Hochberg David Russell Minglei Zhao Arthur Laganowsky |
| author_facet | Jixing Lyu Chang Liu Tianqi Zhang Samantha Schrecke Nicklaus P. Elam Charles Packianathan Georg K. A. Hochberg David Russell Minglei Zhao Arthur Laganowsky |
| author_sort | Jixing Lyu |
| collection | DOAJ |
| description | The bacterial ABC transporter MsbA is essential for lipopolysaccharide biogenesis. Here, the authors apply native mass spectrometry, X-ray crystallography, cryo-EM and biochemical approaches to characterize the structural basis and functional roles of MsbA binding to copper and specific lipids. |
| first_indexed | 2024-04-11T13:53:40Z |
| format | Article |
| id | doaj.art-5e9d0f15449143aebe7c810597dff7e1 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T13:53:40Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-5e9d0f15449143aebe7c810597dff7e12022-12-22T04:20:27ZengNature PortfolioNature Communications2041-17232022-11-0113111110.1038/s41467-022-34905-2Structural basis for lipid and copper regulation of the ABC transporter MsbAJixing Lyu0Chang Liu1Tianqi Zhang2Samantha Schrecke3Nicklaus P. Elam4Charles Packianathan5Georg K. A. Hochberg6David Russell7Minglei Zhao8Arthur Laganowsky9Department of Chemistry, Texas A&M UniversityDepartment of Biochemistry and Molecular biology, University of ChicagoDepartment of Chemistry, Texas A&M UniversityDepartment of Chemistry, Texas A&M UniversityDepartment of Chemistry, Texas A&M UniversityDepartment of Chemistry, Texas A&M UniversityMax Planck Institute for Terrestrial Microbiology and Department of Chemistry, University of MarburgDepartment of Chemistry, Texas A&M UniversityDepartment of Biochemistry and Molecular biology, University of ChicagoDepartment of Chemistry, Texas A&M UniversityThe bacterial ABC transporter MsbA is essential for lipopolysaccharide biogenesis. Here, the authors apply native mass spectrometry, X-ray crystallography, cryo-EM and biochemical approaches to characterize the structural basis and functional roles of MsbA binding to copper and specific lipids.https://doi.org/10.1038/s41467-022-34905-2 |
| spellingShingle | Jixing Lyu Chang Liu Tianqi Zhang Samantha Schrecke Nicklaus P. Elam Charles Packianathan Georg K. A. Hochberg David Russell Minglei Zhao Arthur Laganowsky Structural basis for lipid and copper regulation of the ABC transporter MsbA Nature Communications |
| title | Structural basis for lipid and copper regulation of the ABC transporter MsbA |
| title_full | Structural basis for lipid and copper regulation of the ABC transporter MsbA |
| title_fullStr | Structural basis for lipid and copper regulation of the ABC transporter MsbA |
| title_full_unstemmed | Structural basis for lipid and copper regulation of the ABC transporter MsbA |
| title_short | Structural basis for lipid and copper regulation of the ABC transporter MsbA |
| title_sort | structural basis for lipid and copper regulation of the abc transporter msba |
| url | https://doi.org/10.1038/s41467-022-34905-2 |
| work_keys_str_mv | AT jixinglyu structuralbasisforlipidandcopperregulationoftheabctransportermsba AT changliu structuralbasisforlipidandcopperregulationoftheabctransportermsba AT tianqizhang structuralbasisforlipidandcopperregulationoftheabctransportermsba AT samanthaschrecke structuralbasisforlipidandcopperregulationoftheabctransportermsba AT nicklauspelam structuralbasisforlipidandcopperregulationoftheabctransportermsba AT charlespackianathan structuralbasisforlipidandcopperregulationoftheabctransportermsba AT georgkahochberg structuralbasisforlipidandcopperregulationoftheabctransportermsba AT davidrussell structuralbasisforlipidandcopperregulationoftheabctransportermsba AT mingleizhao structuralbasisforlipidandcopperregulationoftheabctransportermsba AT arthurlaganowsky structuralbasisforlipidandcopperregulationoftheabctransportermsba |