Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model
Advanced glycation end-products (AGEs) play a vital role in the pathogenesis of diabetic complications. Methylglyoxal (MGO), one of the major precursors of AGEs, is a highly reactive dicarbonyl compound that plays an important role in the pathogenesis of diabetic nephropathy. This study was designed...
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MDPI AG
2022-05-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/6/355 |
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| author | Chi-Heung Cho Chang-Jun Lee Min-Gyeong Kim Bomi Ryu Jun-Geon Je Yoonsook Kim Sang-Hoon Lee |
| author_facet | Chi-Heung Cho Chang-Jun Lee Min-Gyeong Kim Bomi Ryu Jun-Geon Je Yoonsook Kim Sang-Hoon Lee |
| author_sort | Chi-Heung Cho |
| collection | DOAJ |
| description | Advanced glycation end-products (AGEs) play a vital role in the pathogenesis of diabetic complications. Methylglyoxal (MGO), one of the major precursors of AGEs, is a highly reactive dicarbonyl compound that plays an important role in the pathogenesis of diabetic nephropathy. This study was designed to evaluate the therapeutic potential of phlorotannin-rich <i>Ecklonia cava</i> extract (ECE) on MGO-induced diabetic nephropathy in in vitro models using mouse glomerular mesangial cells. ECE showed anti-glycation activity via breaking of AGEs-collagen cross-links and inhibition of AGEs formation and AGE-collagen cross-linking formation. The renoprotective effects were determined by assessing intracellular reactive oxygen species (ROS) and MGO accumulation, cell apoptosis, and the Nrf-2/ARE signaling pathway. MGO-induced renal damage, intracellular ROS production level, and MGO-protein adduct accumulation were significantly decreased by pretreating ECE. Moreover, ECE pretreatment exhibited preventive properties against MGO-induced dicarbonyl stress via activation of the Nrf2/ARE signaling pathway and reduction of RAGE protein expression in mouse glomerular mesangial cells. Collectively, these results indicated potential anti-glycation properties and prominent preventive effects of ECE against MGO-induced renal damage. Additionally, ECE may be utilized for the management of AGE-related diabetic nephropathy. |
| first_indexed | 2024-03-09T23:13:37Z |
| format | Article |
| id | doaj.art-5ea389809dd24fdf87cfc690c43921df |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T23:13:37Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-5ea389809dd24fdf87cfc690c43921df2023-11-23T17:40:48ZengMDPI AGMarine Drugs1660-33972022-05-0120635510.3390/md20060355Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro ModelChi-Heung Cho0Chang-Jun Lee1Min-Gyeong Kim2Bomi Ryu3Jun-Geon Je4Yoonsook Kim5Sang-Hoon Lee6Division of Functional Food Research, Korea Food Research Institute, Wanju 55365, KoreaDivision of Functional Food Research, Korea Food Research Institute, Wanju 55365, KoreaDivision of Functional Food Research, Korea Food Research Institute, Wanju 55365, KoreaMarine Science Institute, Jeju National University, Jeju 63333, KoreaMarine Science Institute, Jeju National University, Jeju 63333, KoreaDivision of Functional Food Research, Korea Food Research Institute, Wanju 55365, KoreaDivision of Functional Food Research, Korea Food Research Institute, Wanju 55365, KoreaAdvanced glycation end-products (AGEs) play a vital role in the pathogenesis of diabetic complications. Methylglyoxal (MGO), one of the major precursors of AGEs, is a highly reactive dicarbonyl compound that plays an important role in the pathogenesis of diabetic nephropathy. This study was designed to evaluate the therapeutic potential of phlorotannin-rich <i>Ecklonia cava</i> extract (ECE) on MGO-induced diabetic nephropathy in in vitro models using mouse glomerular mesangial cells. ECE showed anti-glycation activity via breaking of AGEs-collagen cross-links and inhibition of AGEs formation and AGE-collagen cross-linking formation. The renoprotective effects were determined by assessing intracellular reactive oxygen species (ROS) and MGO accumulation, cell apoptosis, and the Nrf-2/ARE signaling pathway. MGO-induced renal damage, intracellular ROS production level, and MGO-protein adduct accumulation were significantly decreased by pretreating ECE. Moreover, ECE pretreatment exhibited preventive properties against MGO-induced dicarbonyl stress via activation of the Nrf2/ARE signaling pathway and reduction of RAGE protein expression in mouse glomerular mesangial cells. Collectively, these results indicated potential anti-glycation properties and prominent preventive effects of ECE against MGO-induced renal damage. Additionally, ECE may be utilized for the management of AGE-related diabetic nephropathy.https://www.mdpi.com/1660-3397/20/6/355<i>Ecklonia cava</i>phlorotannindiabetic nephropathyadvanced glycation end-productsmethylglyoxalmouse glomerular mesangial cell |
| spellingShingle | Chi-Heung Cho Chang-Jun Lee Min-Gyeong Kim Bomi Ryu Jun-Geon Je Yoonsook Kim Sang-Hoon Lee Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model Marine Drugs <i>Ecklonia cava</i> phlorotannin diabetic nephropathy advanced glycation end-products methylglyoxal mouse glomerular mesangial cell |
| title | Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model |
| title_full | Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model |
| title_fullStr | Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model |
| title_full_unstemmed | Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model |
| title_short | Therapeutic Potential of Phlorotannin-Rich <i>Ecklonia cava</i> Extract on Methylglyoxal-Induced Diabetic Nephropathy in In Vitro Model |
| title_sort | therapeutic potential of phlorotannin rich i ecklonia cava i extract on methylglyoxal induced diabetic nephropathy in in vitro model |
| topic | <i>Ecklonia cava</i> phlorotannin diabetic nephropathy advanced glycation end-products methylglyoxal mouse glomerular mesangial cell |
| url | https://www.mdpi.com/1660-3397/20/6/355 |
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