Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease

Summary: In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model...

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Bibliographic Details
Main Authors: Suzana Ulian-Benitez, Abigail Strausbaugh Hjelmstad, Guilherme Oliveira Barbosa, Pejmun Haghighi
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200257X