Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease
Summary: In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200257X |
| _version_ | 1828748208725032960 |
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| author | Suzana Ulian-Benitez Abigail Strausbaugh Hjelmstad Guilherme Oliveira Barbosa Pejmun Haghighi |
| author_facet | Suzana Ulian-Benitez Abigail Strausbaugh Hjelmstad Guilherme Oliveira Barbosa Pejmun Haghighi |
| author_sort | Suzana Ulian-Benitez |
| collection | DOAJ |
| description | Summary: In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzheimer disease. We describe eosin staining in the whole-brain mount, followed by confocal microscopy and image analysis with an open source Fiji plugin. This protocol can be applied to visualize different modules in the fly brain. |
| first_indexed | 2024-04-14T04:57:53Z |
| format | Article |
| id | doaj.art-5ea826a81b1c477ab6d69ccaff9900c5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-14T04:57:53Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-5ea826a81b1c477ab6d69ccaff9900c52022-12-22T02:11:06ZengElsevierSTAR Protocols2666-16672022-06-0132101377Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s diseaseSuzana Ulian-Benitez0Abigail Strausbaugh Hjelmstad1Guilherme Oliveira Barbosa2Pejmun Haghighi3Buck Institute for Research on Aging, Novato, CA 94945, USA; Corresponding authorBuck Institute for Research on Aging, Novato, CA 94945, USAUniversity California San Francisco, San Francisco, CA 94158, USABuck Institute for Research on Aging, Novato, CA 94945, USA; Corresponding authorSummary: In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzheimer disease. We describe eosin staining in the whole-brain mount, followed by confocal microscopy and image analysis with an open source Fiji plugin. This protocol can be applied to visualize different modules in the fly brain.http://www.sciencedirect.com/science/article/pii/S266616672200257XCell BiologyMicroscopyModel OrganismsNeuroscience |
| spellingShingle | Suzana Ulian-Benitez Abigail Strausbaugh Hjelmstad Guilherme Oliveira Barbosa Pejmun Haghighi Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease STAR Protocols Cell Biology Microscopy Model Organisms Neuroscience |
| title | Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease |
| title_full | Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease |
| title_fullStr | Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease |
| title_full_unstemmed | Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease |
| title_short | Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer’s disease |
| title_sort | eosin whole brain mount staining to analyze neurodegeneration in a fly model of alzheimer s disease |
| topic | Cell Biology Microscopy Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S266616672200257X |
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