DNA elution from buccal cells stored on Whatman FTA Classic Cards using a modified methanol fixation method

We describe here a method for DNA elution from buccal cells and whole blood both collected onto Whatman FTA technology, using methanol fixation followed by an elution PCR program. Extracted DNA is comparable in quality to published Whatman FTA protocols, as judged by PCR-based genotyping. Elution of...

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Detalhes bibliográficos
Principais autores: Helene C. Johanson, Valentine Hyland, Carol Wicking, Richard A. Sturm
Formato: Artigo
Idioma:English
Publicado em: Taylor & Francis Group 2009-04-01
coleção:BioTechniques
Assuntos:
Acesso em linha:https://www.future-science.com/doi/10.2144/000113077