Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin
Adiponectin exhibits potent antitumor activities. Herein, we examined the molecular mechanisms underlying suppression of tumor growth by globular adiponectin (gAcrp). We demonstrated that gAcrp suppressed B‐cell lymphoma 2 (Bcl‐2) expression, an anti‐apoptotic gene, by inducing its mRNA destabilizat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-12-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12541 |
| _version_ | 1797871004693823488 |
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| author | Nirmala Tilija Pun Amrita Khakurel Aastha Shrestha Sang‐Hyun Kim Pil‐Hoon Park |
| author_facet | Nirmala Tilija Pun Amrita Khakurel Aastha Shrestha Sang‐Hyun Kim Pil‐Hoon Park |
| author_sort | Nirmala Tilija Pun |
| collection | DOAJ |
| description | Adiponectin exhibits potent antitumor activities. Herein, we examined the molecular mechanisms underlying suppression of tumor growth by globular adiponectin (gAcrp). We demonstrated that gAcrp suppressed B‐cell lymphoma 2 (Bcl‐2) expression, an anti‐apoptotic gene, by inducing its mRNA destabilization, which was accompanied with a decrease in cell viability and increased caspase‐3 activity in hepatic cancer cells. In addition, gAcrp increased expression of tristetraprolin (TTP) and AU‐rich element RNA‐binding protein 1 (AUF1), which are mRNA stability regulatory proteins. Moreover, gAcrp‐induced suppression of Bcl‐2 expression was abrogated by knockdown of TTP or AUF1. These data indicate that gAcrp induces apoptosis of hepatic cancer cells by TTP‐ and AUF1‐mediated Bcl‐2 mRNA destabilization, and further suggest that TTP and AUF1 are novel targets mediating the antitumor activity of adiponectin. |
| first_indexed | 2024-04-10T00:37:23Z |
| format | Article |
| id | doaj.art-5eae0806083b45668ef5b9336798c0fa |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-10T00:37:23Z |
| publishDate | 2018-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-5eae0806083b45668ef5b9336798c0fa2023-03-14T13:06:01ZengWileyFEBS Open Bio2211-54632018-12-018121964197610.1002/2211-5463.12541Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectinNirmala Tilija Pun0Amrita Khakurel1Aastha Shrestha2Sang‐Hyun Kim3Pil‐Hoon Park4College of Pharmacy Yeungnam University Gyeongsan KoreaCollege of Pharmacy Yeungnam University Gyeongsan KoreaCollege of Pharmacy Yeungnam University Gyeongsan KoreaDepartment of Pharmacology School of Medicine Kyungpook National University Daegu KoreaCollege of Pharmacy Yeungnam University Gyeongsan KoreaAdiponectin exhibits potent antitumor activities. Herein, we examined the molecular mechanisms underlying suppression of tumor growth by globular adiponectin (gAcrp). We demonstrated that gAcrp suppressed B‐cell lymphoma 2 (Bcl‐2) expression, an anti‐apoptotic gene, by inducing its mRNA destabilization, which was accompanied with a decrease in cell viability and increased caspase‐3 activity in hepatic cancer cells. In addition, gAcrp increased expression of tristetraprolin (TTP) and AU‐rich element RNA‐binding protein 1 (AUF1), which are mRNA stability regulatory proteins. Moreover, gAcrp‐induced suppression of Bcl‐2 expression was abrogated by knockdown of TTP or AUF1. These data indicate that gAcrp induces apoptosis of hepatic cancer cells by TTP‐ and AUF1‐mediated Bcl‐2 mRNA destabilization, and further suggest that TTP and AUF1 are novel targets mediating the antitumor activity of adiponectin.https://doi.org/10.1002/2211-5463.12541adiponectinAUF1Bcl‐2hepatic cancerTTP |
| spellingShingle | Nirmala Tilija Pun Amrita Khakurel Aastha Shrestha Sang‐Hyun Kim Pil‐Hoon Park Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin FEBS Open Bio adiponectin AUF1 Bcl‐2 hepatic cancer TTP |
| title | Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin |
| title_full | Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin |
| title_fullStr | Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin |
| title_full_unstemmed | Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin |
| title_short | Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin |
| title_sort | critical role of tristetraprolin and au rich element rna binding protein 1 in the suppression of cancer cell growth by globular adiponectin |
| topic | adiponectin AUF1 Bcl‐2 hepatic cancer TTP |
| url | https://doi.org/10.1002/2211-5463.12541 |
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