A novel genotyping method to determine copy number in a mouse line commonly used for inducible transgene expression in brain and spinal cord [version 2; peer review: 1 approved, 2 approved with reservations]
Background: The NEFH-tTA mouse has the human neurofilament heavy polypeptide promoter directing tetracycline-controlled transactivator protein (tTa) expression to the brain and spinal cord, allowing tissue-specific and doxycycline-suppressible expression of a target gene. Current genotyping protocol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2022-02-01
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| Series: | F1000Research |
| Subjects: | |
| Online Access: | https://f1000research.com/articles/9-1249/v2 |