Compact CRISPR genetic screens enabled by improved guide RNA library cloning

Compact CRISPR genetic screens enabled by improved guide RNA library cloning

Abstract CRISPR genome editing approaches theoretically enable researchers to define the function of each human gene in specific cell types, but challenges remain to efficiently perform genetic perturbations in relevant models. In this work, we develop a library cloning protocol that increases sgRNA...

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Bibliographic Details
Main Authors: Seok-Jin Heo, Lauren D. Enriquez, Scot Federman, Amy Y. Chang, Rachel Mace, Kaivalya Shevade, Phuong Nguyen, Adam J. Litterman, Shawn Shafer, Laralynne Przybyla, Eric D. Chow
Format: Article
Language:English
Published: BMC 2024-01-01
Series:Genome Biology
Subjects:
CRISPR screening
Library cloning
Guide library
iPSC-derived CRISPR screening
Primary cell CRISPR screening
Online Access:https://doi.org/10.1186/s13059-023-03132-3

Internet

https://doi.org/10.1186/s13059-023-03132-3

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