Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative Colitis
Background: The pathogenesis of ulcerative colitis (UC) is closely related to immunity. The immune characteristic differences between active UC (UCa) and inactive UC (UCin) have not been completely explained. Mass cytometry (CyTOF) and single-cell RNA sequencing (scRNA-seq) were used to analyze the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.859645/full |
| _version_ | 1811335184072572928 |
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| author | Yongxin Luo Shiying Liu Huibiao Li Jiangtao Hou Wenjia Lin Zewen Xu Tianyu Lu Yanwu Li Yanwu Li Bin Peng Shijing Zhang Xue Han Zuoliang Kuang Yi Wen Jiazhong Cai Jiazhong Cai Fengbin Liu Xin-Lin Chen |
| author_facet | Yongxin Luo Shiying Liu Huibiao Li Jiangtao Hou Wenjia Lin Zewen Xu Tianyu Lu Yanwu Li Yanwu Li Bin Peng Shijing Zhang Xue Han Zuoliang Kuang Yi Wen Jiazhong Cai Jiazhong Cai Fengbin Liu Xin-Lin Chen |
| author_sort | Yongxin Luo |
| collection | DOAJ |
| description | Background: The pathogenesis of ulcerative colitis (UC) is closely related to immunity. The immune characteristic differences between active UC (UCa) and inactive UC (UCin) have not been completely explained. Mass cytometry (CyTOF) and single-cell RNA sequencing (scRNA-seq) were used to analyze the immune cells of UCa, UCin and healthy control (HC) subjects to determine the specific immune characteristics.Methods: The immune cell subsets among UCa, UCin, HC were distinguished using CyTOF analysis. scRNA-seq analysis was used to validate the results of CyTOF. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to understand the roles of differential immune cell subsets.Results: After CyTOF analysis and validation of scRNA-seq analysis, differential immune cell subsets mainly contained TNF+IL-17A++ effector memory (EM) Tregs, CXCR3+CTLA4+ EM Tregs, CXCR3++CCR7+ B cells, HLA-DR+CCR7+ dendritic cells (DCs) and CTLA-4+ natural killer (NK) cells. In comparison to HC, CCR6+TNF+CD161+ EM T cells were highly enriched in UCa and UCin. Besides, UCa was characterized by an increase in CD38+TNF+ EM Tregs, CXCR3+CCR4+ naïve B cells, HLA-DR+CD14+IL21+ macrophages/monocytes, HLA-DR+CCR7+ DCs, AHR+CD14+ cytotoxic NK (cNK) cells and CD8A+IFNG+ cNK cells. Decreases in CD38+CD27+ plasmablasts, CXCR3+CD38+ regulatory NK cells, and CXCR3+CCR7+ tolerant NK cells in UCa were discovered.Conclusions: Novel immune cell subsets which was used to distinguish UCa, UCin and HC were identified. This information might be utilized to distinguish the patients with UCa and UCin. |
| first_indexed | 2024-04-13T17:20:24Z |
| format | Article |
| id | doaj.art-5f04cef77b264171a9f3680b69394664 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-13T17:20:24Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-5f04cef77b264171a9f3680b693946642022-12-22T02:38:00ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.859645859645Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative ColitisYongxin Luo0Shiying Liu1Huibiao Li2Jiangtao Hou3Wenjia Lin4Zewen Xu5Tianyu Lu6Yanwu Li7Yanwu Li8Bin Peng9Shijing Zhang10Xue Han11Zuoliang Kuang12Yi Wen13Jiazhong Cai14Jiazhong Cai15Fengbin Liu16Xin-Lin Chen17School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaScience and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, ChinaScience and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaBackground: The pathogenesis of ulcerative colitis (UC) is closely related to immunity. The immune characteristic differences between active UC (UCa) and inactive UC (UCin) have not been completely explained. Mass cytometry (CyTOF) and single-cell RNA sequencing (scRNA-seq) were used to analyze the immune cells of UCa, UCin and healthy control (HC) subjects to determine the specific immune characteristics.Methods: The immune cell subsets among UCa, UCin, HC were distinguished using CyTOF analysis. scRNA-seq analysis was used to validate the results of CyTOF. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to understand the roles of differential immune cell subsets.Results: After CyTOF analysis and validation of scRNA-seq analysis, differential immune cell subsets mainly contained TNF+IL-17A++ effector memory (EM) Tregs, CXCR3+CTLA4+ EM Tregs, CXCR3++CCR7+ B cells, HLA-DR+CCR7+ dendritic cells (DCs) and CTLA-4+ natural killer (NK) cells. In comparison to HC, CCR6+TNF+CD161+ EM T cells were highly enriched in UCa and UCin. Besides, UCa was characterized by an increase in CD38+TNF+ EM Tregs, CXCR3+CCR4+ naïve B cells, HLA-DR+CD14+IL21+ macrophages/monocytes, HLA-DR+CCR7+ DCs, AHR+CD14+ cytotoxic NK (cNK) cells and CD8A+IFNG+ cNK cells. Decreases in CD38+CD27+ plasmablasts, CXCR3+CD38+ regulatory NK cells, and CXCR3+CCR7+ tolerant NK cells in UCa were discovered.Conclusions: Novel immune cell subsets which was used to distinguish UCa, UCin and HC were identified. This information might be utilized to distinguish the patients with UCa and UCin.https://www.frontiersin.org/articles/10.3389/fmolb.2022.859645/fullulcerative colitisCyTOFscRNA-seq analysisimmune cellsTregs |
| spellingShingle | Yongxin Luo Shiying Liu Huibiao Li Jiangtao Hou Wenjia Lin Zewen Xu Tianyu Lu Yanwu Li Yanwu Li Bin Peng Shijing Zhang Xue Han Zuoliang Kuang Yi Wen Jiazhong Cai Jiazhong Cai Fengbin Liu Xin-Lin Chen Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative Colitis Frontiers in Molecular Biosciences ulcerative colitis CyTOF scRNA-seq analysis immune cells Tregs |
| title | Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative Colitis |
| title_full | Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative Colitis |
| title_fullStr | Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative Colitis |
| title_full_unstemmed | Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative Colitis |
| title_short | Mass Cytometry and Single-Cell Transcriptome Analyses Reveal the Immune Cell Characteristics of Ulcerative Colitis |
| title_sort | mass cytometry and single cell transcriptome analyses reveal the immune cell characteristics of ulcerative colitis |
| topic | ulcerative colitis CyTOF scRNA-seq analysis immune cells Tregs |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.859645/full |
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