Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes

The BioID approaches takes advantage of the promiscuous biotinylation enzyme (BirA*) to identify proteins that closely interact. Here the authors improve the resolution of BioID using a protein fragment complementation approach that allows the assignment of protein-protein interactions to specific c...

Full description

Bibliographic Details
Main Authors: Isabel Myriam Schopp, Cinthia Claudia Amaya Ramirez, Jerneja Debeljak, Elisa Kreibich, Merle Skribbe, Klemens Wild, Julien Béthune
Format: Article
Language:English
Published: Nature Portfolio 2017-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15690