Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

Abstract Reverse transcription quantitative PCR (RT-qPCR) assays are gold standard in diagnosing SARS-CoV-2 infection and play a major role in viral subtyping for rapid detection and monitoring of important mutations, containing the spread of new virus variants. We wanted to compare RT-qPCR melting...

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Bibliographic Details
Main Authors: Sebastian Juul, Malene Roed Spiegelhauer, Mette Neve Petersen, Katharina Kirkegaard Flugt, Nikolaj Vestergaard Hansen, Helene Larsen, Per Bo Jensen, Ulf Bech Christensen, Rasmus Koefoed Petersen, Lennart Friis-Hansen
Format: Article
Language:English
Published: Nature Portfolio 2022-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-17339-0

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https://doi.org/10.1038/s41598-022-17339-0

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