A new and efficient enrichment method for metagenomic sequencing of Monkeypox virus

A new and efficient enrichment method for metagenomic sequencing of Monkeypox virus

Abstract Background The methodology described in previous literature for Monkeypox virus (MPXV) sequencing shows low efficiency when using metagenomic approaches. The aim of the present study was to evaluate a new fine-tuned method for extraction and enrichment of genomic MPXV DNA using clinical sam...

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Bibliographic Details
Main Authors: Pablo Aja-Macaya, Soraya Rumbo-Feal, Margarita Poza, Angelina Cañizares, Juan A. Vallejo, Germán Bou
Format: Article
Language:English
Published: BMC 2023-01-01
Series:BMC Genomics
Subjects:
Human monkeypox (hMPX)
Monkeypox virus (MPXV)
Genome sequencing
Viral surveillance
Metagenomics
Host DNA depletion
Online Access:https://doi.org/10.1186/s12864-023-09114-w

Internet

https://doi.org/10.1186/s12864-023-09114-w

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