MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway.
Currently, there are difficulties associated with the culturing of pluripotent human embryonic stem cells (hESCs), and knowledge regarding their regulatory mechanisms is limited. MicroRNAs (miRNAs) regulate gene expression and have critical functions in stem cell self-renewal and differentiation. Mo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3826748?pdf=render |
| _version_ | 1828200317097869312 |
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| author | Yan-Hui Yu Li Zhang Deng-Shu Wu Zheng Zhang Fang-Fang Huang Jian Zhang Xiao-Ping Chen De-Sheng Liang Hui Zeng Fang-Ping Chen |
| author_facet | Yan-Hui Yu Li Zhang Deng-Shu Wu Zheng Zhang Fang-Fang Huang Jian Zhang Xiao-Ping Chen De-Sheng Liang Hui Zeng Fang-Ping Chen |
| author_sort | Yan-Hui Yu |
| collection | DOAJ |
| description | Currently, there are difficulties associated with the culturing of pluripotent human embryonic stem cells (hESCs), and knowledge regarding their regulatory mechanisms is limited. MicroRNAs (miRNAs) regulate gene expression and have critical functions in stem cell self-renewal and differentiation. Moreover, fibroblast growth factor (FGF) and the insulin-like growth factor receptor (IGF-1R) are key activators of signaling in hESCs. Based on the identification of complementary binding sites in miR-223 and IGF-1R mRNA, it is proposed that miR-223 acts as a local regulator of IGF-1R. Therefore, levels of miR-223 were detected in differentiated versus undifferentiated hESCs. In addition, proliferation, apoptosis, and differentiation were assayed in these two hESC populations and were compared in the presence of exogenous miR-223 and miR-223 inhibitor. Inhibition of miR-223 was found to maintain the undifferentiated state of hESCs, while addition of miR-223 induced differentiation. Furthermore, these effects were found to be likely dependent on IGF-1R/Akt signaling. |
| first_indexed | 2024-04-12T11:11:15Z |
| format | Article |
| id | doaj.art-5f44f6bbed0d4eb6bbe33c49c6677110 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:11:15Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5f44f6bbed0d4eb6bbe33c49c66771102022-12-22T03:35:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7876910.1371/journal.pone.0078769MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway.Yan-Hui YuLi ZhangDeng-Shu WuZheng ZhangFang-Fang HuangJian ZhangXiao-Ping ChenDe-Sheng LiangHui ZengFang-Ping ChenCurrently, there are difficulties associated with the culturing of pluripotent human embryonic stem cells (hESCs), and knowledge regarding their regulatory mechanisms is limited. MicroRNAs (miRNAs) regulate gene expression and have critical functions in stem cell self-renewal and differentiation. Moreover, fibroblast growth factor (FGF) and the insulin-like growth factor receptor (IGF-1R) are key activators of signaling in hESCs. Based on the identification of complementary binding sites in miR-223 and IGF-1R mRNA, it is proposed that miR-223 acts as a local regulator of IGF-1R. Therefore, levels of miR-223 were detected in differentiated versus undifferentiated hESCs. In addition, proliferation, apoptosis, and differentiation were assayed in these two hESC populations and were compared in the presence of exogenous miR-223 and miR-223 inhibitor. Inhibition of miR-223 was found to maintain the undifferentiated state of hESCs, while addition of miR-223 induced differentiation. Furthermore, these effects were found to be likely dependent on IGF-1R/Akt signaling.http://europepmc.org/articles/PMC3826748?pdf=render |
| spellingShingle | Yan-Hui Yu Li Zhang Deng-Shu Wu Zheng Zhang Fang-Fang Huang Jian Zhang Xiao-Ping Chen De-Sheng Liang Hui Zeng Fang-Ping Chen MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway. PLoS ONE |
| title | MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway. |
| title_full | MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway. |
| title_fullStr | MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway. |
| title_full_unstemmed | MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway. |
| title_short | MiR-223 regulates human embryonic stem cell differentiation by targeting the IGF-1R/Akt signaling pathway. |
| title_sort | mir 223 regulates human embryonic stem cell differentiation by targeting the igf 1r akt signaling pathway |
| url | http://europepmc.org/articles/PMC3826748?pdf=render |
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