Characterization of N-Terminal Asparagine Deamidation and Clipping of a Monoclonal Antibody

Characterization of N-Terminal Asparagine Deamidation and Clipping of a Monoclonal Antibody

This study presents a novel degradation pathway of a human immunoglobulin G (IgG) molecule featuring a light chain N-terminal asparagine. We thoroughly characterize this pathway and investigate its charge profiles using cation exchange chromatography (CEX) and capillary isoelectric focusing (cIEF)....

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Bibliographic Details
Main Authors: Jing Zhen, Jennifer Lee, Yueyang Wang, Lena McLaughlin, Fei Yang, Zhengjian Li, Jihong Wang
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:Antibodies
Subjects:
monoclonal antibody(s)
biopharmaceutical characterization
deamidation
N-terminal truncation
charge profile
liquid chromatography–mass spectrometry (LC-MS)
Online Access:https://www.mdpi.com/2073-4468/12/3/59

Internet

https://www.mdpi.com/2073-4468/12/3/59

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