Rapid and reliable RNA resonance assignment by combining chemical and enzymatic stable isotope labeling

In this work a rapid RNA assignment approach by combining chemical and enzymatic 13C and 15N stable isotope labeling is introduced. We exemplify the assignment strategy for imino N1H1 purine and N3H3 pyrimidine and aromatic C6H6 pyrimidine, C8H8 purine and C2H2 adenine resonances for a non-coding RN...

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Bibliographic Details
Main Authors: David Klingler, Matthias Huber, Martin Tollinger, Christoph Kreutz
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:Journal of Magnetic Resonance Open
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666441022000474