Rapid and reliable RNA resonance assignment by combining chemical and enzymatic stable isotope labeling
In this work a rapid RNA assignment approach by combining chemical and enzymatic 13C and 15N stable isotope labeling is introduced. We exemplify the assignment strategy for imino N1H1 purine and N3H3 pyrimidine and aromatic C6H6 pyrimidine, C8H8 purine and C2H2 adenine resonances for a non-coding RN...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-12-01
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Series: | Journal of Magnetic Resonance Open |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666441022000474 |