Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency
Numerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different <i>Bacillus</i> strains. Some <i>Bacillus</i> strains have a distinctive predominance and are widely recognized among other microbial communities, as a result of their var...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/7/3210 |
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| author | Moldir Koilybayeva Zhanserik Shynykul Gulbaram Ustenova Symbat Abzaliyeva Mereke Alimzhanova Akerke Amirkhanova Aknur Turgumbayeva Kamilya Mustafina Gulnur Yeleken Karlygash Raganina Elmira Kapsalyamova |
| author_facet | Moldir Koilybayeva Zhanserik Shynykul Gulbaram Ustenova Symbat Abzaliyeva Mereke Alimzhanova Akerke Amirkhanova Aknur Turgumbayeva Kamilya Mustafina Gulnur Yeleken Karlygash Raganina Elmira Kapsalyamova |
| author_sort | Moldir Koilybayeva |
| collection | DOAJ |
| description | Numerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different <i>Bacillus</i> strains. Some <i>Bacillus</i> strains have a distinctive predominance and are widely recognized among other microbial communities, as a result of their varied habitation and physiologically active metabolites. The present study collected vegetable products (potato, carrot, and tomato) from local markets in Almaty, Kazakhstan. The bacterial isolates were identified using biochemical and phylogenetic analyses after culturing. Our phylogenetic analysis revealed three Gram-positive bacterial isolates BSS11, BSS17, and BSS19 showing 99% nucleotide sequence similarities with <i>Bacillus subtilis</i> O-3, <i>Bacillus subtilis</i> Md1-42, and <i>Bacillus subtilis</i> Khozestan2. The crude extract was prepared from bacterial isolates to assess the antibiotic resistance potency and the antimicrobial potential against various targeted multidrug-resistant strains, including <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, <i>Streptococcus group B</i>, <i>Streptococcus mutans</i>, <i>Candida albicans</i>, <i>Candida krusei</i>, <i>Pseudomonas aeruginosa</i>, <i>Shigella sonnei</i>, <i>Klebsiella pneumoniae</i>, <i>Salmonella enteritidis</i>, <i>Klebsiella aerogenes</i>, <i>Enterococcus hirae</i>, <i>Escherichia coli</i>, <i>Serratia marcescens</i>, and <i>Proteus vulgaris</i>. This study found that the species that were identified have the ability to produce antibiotic chemicals. Additionally, the GC–MS analysis of three bacterial extracts revealed the presence of many antibiotic substances including phenol, benzoic acid, 1,2-benzenedicarboxylic acid and bis(2-methylpropyl), methoxyphenyl-oxime, and benzaldehyde. This work sheds light on the potential of <i>Bacillus</i> to be employed as an antimicrobial agent to target different multidrug-resistant bacterial strains. The results indicate that market vegetables may be a useful source of strains displaying a range of advantageous characteristics that can be used in the creation of biological antibiotics. |
| first_indexed | 2024-03-11T05:29:40Z |
| format | Article |
| id | doaj.art-5fa5de589fb34edb880e47e534799168 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T05:29:40Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5fa5de589fb34edb880e47e5347991682023-11-17T17:15:09ZengMDPI AGMolecules1420-30492023-04-01287321010.3390/molecules28073210Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic PotencyMoldir Koilybayeva0Zhanserik Shynykul1Gulbaram Ustenova2Symbat Abzaliyeva3Mereke Alimzhanova4Akerke Amirkhanova5Aknur Turgumbayeva6Kamilya Mustafina7Gulnur Yeleken8Karlygash Raganina9Elmira Kapsalyamova10School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, KazakhstanHigher School of Medicine, Al-Farabi Kazakh National University, Tole-bi 96, Almaty 050040, KazakhstanSchool of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, KazakhstanHigher School of Medicine, Al-Farabi Kazakh National University, Tole-bi 96, Almaty 050040, KazakhstanCenter of Physical Chemical Methods of Research and Analysis, Al-Farabi Kazakh National University, Tole-bi 96, Almaty 050012, KazakhstanSchool of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, KazakhstanHigher School of Medicine, Al-Farabi Kazakh National University, Tole-bi 96, Almaty 050040, KazakhstanSchool of Medicine, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, KazakhstanSchool of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, KazakhstanSchool of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, KazakhstanSchool of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty 050012, KazakhstanNumerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different <i>Bacillus</i> strains. Some <i>Bacillus</i> strains have a distinctive predominance and are widely recognized among other microbial communities, as a result of their varied habitation and physiologically active metabolites. The present study collected vegetable products (potato, carrot, and tomato) from local markets in Almaty, Kazakhstan. The bacterial isolates were identified using biochemical and phylogenetic analyses after culturing. Our phylogenetic analysis revealed three Gram-positive bacterial isolates BSS11, BSS17, and BSS19 showing 99% nucleotide sequence similarities with <i>Bacillus subtilis</i> O-3, <i>Bacillus subtilis</i> Md1-42, and <i>Bacillus subtilis</i> Khozestan2. The crude extract was prepared from bacterial isolates to assess the antibiotic resistance potency and the antimicrobial potential against various targeted multidrug-resistant strains, including <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, <i>Streptococcus group B</i>, <i>Streptococcus mutans</i>, <i>Candida albicans</i>, <i>Candida krusei</i>, <i>Pseudomonas aeruginosa</i>, <i>Shigella sonnei</i>, <i>Klebsiella pneumoniae</i>, <i>Salmonella enteritidis</i>, <i>Klebsiella aerogenes</i>, <i>Enterococcus hirae</i>, <i>Escherichia coli</i>, <i>Serratia marcescens</i>, and <i>Proteus vulgaris</i>. This study found that the species that were identified have the ability to produce antibiotic chemicals. Additionally, the GC–MS analysis of three bacterial extracts revealed the presence of many antibiotic substances including phenol, benzoic acid, 1,2-benzenedicarboxylic acid and bis(2-methylpropyl), methoxyphenyl-oxime, and benzaldehyde. This work sheds light on the potential of <i>Bacillus</i> to be employed as an antimicrobial agent to target different multidrug-resistant bacterial strains. The results indicate that market vegetables may be a useful source of strains displaying a range of advantageous characteristics that can be used in the creation of biological antibiotics.https://www.mdpi.com/1420-3049/28/7/3210antimicrobial activityantibiotic substances<i>Bacillus subtilis</i><i>Bacillus subtilis</i>Khozestan2bacterial isolates |
| spellingShingle | Moldir Koilybayeva Zhanserik Shynykul Gulbaram Ustenova Symbat Abzaliyeva Mereke Alimzhanova Akerke Amirkhanova Aknur Turgumbayeva Kamilya Mustafina Gulnur Yeleken Karlygash Raganina Elmira Kapsalyamova Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency Molecules antimicrobial activity antibiotic substances <i>Bacillus subtilis</i> <i>Bacillus subtilis</i> Khozestan2 bacterial isolates |
| title | Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency |
| title_full | Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency |
| title_fullStr | Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency |
| title_full_unstemmed | Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency |
| title_short | Molecular Characterization of Some <i>Bacillus</i> Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency |
| title_sort | molecular characterization of some i bacillus i species from vegetables and evaluation of their antimicrobial and antibiotic potency |
| topic | antimicrobial activity antibiotic substances <i>Bacillus subtilis</i> <i>Bacillus subtilis</i> Khozestan2 bacterial isolates |
| url | https://www.mdpi.com/1420-3049/28/7/3210 |
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