Functional Validation of <i>cas9</i>/GuideRNA Constructs for Site-Directed Mutagenesis of Triticale <i>ABA8′OH1 loci</i>

Functional Validation of <i>cas9</i>/GuideRNA Constructs for Site-Directed Mutagenesis of Triticale <i>ABA8′OH1 loci</i>

Cas endonuclease-mediated genome editing provides a long-awaited molecular biological approach to the modification of predefined genomic target sequences in living organisms. Although <i>cas9</i>/guide (g)RNA constructs are straightforward to assemble and can be customized to target virt...

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Bibliographic Details
Main Authors: Krzysztof Michalski, Christian Hertig, Dariusz R. Mańkowski, Jochen Kumlehn, Janusz Zimny, Anna M. Linkiewicz
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:International Journal of Molecular Sciences
Subjects:
genome editing
CRISPR
protoplasts
targeted mutagenesis
TREX2
construct validation
Online Access:https://www.mdpi.com/1422-0067/22/13/7038

Internet

https://www.mdpi.com/1422-0067/22/13/7038

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