Functional Validation of <i>cas9</i>/GuideRNA Constructs for Site-Directed Mutagenesis of Triticale <i>ABA8′OH1 loci</i>
Cas endonuclease-mediated genome editing provides a long-awaited molecular biological approach to the modification of predefined genomic target sequences in living organisms. Although <i>cas9</i>/guide (g)RNA constructs are straightforward to assemble and can be customized to target virt...
Main Authors: | Krzysztof Michalski, Christian Hertig, Dariusz R. Mańkowski, Jochen Kumlehn, Janusz Zimny, Anna M. Linkiewicz |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-06-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/13/7038 |
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