Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins. Purification methods for recombinant proteins containing His-tags are relatively well-established, thus His-tags are widely used in pr...

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Bibliographic Details
Main Authors: He Shuzhen, Xu Ruixian, Yi Huashan, Chen Zhixin, Chen Congjie, Li Qiang, Han Qinqin, Xia Xueshan, Song Yuzhu, Xu Junwei, Zhang Jinyang
Format: Article
Language:English
Published: De Gruyter 2022-11-01
Series:Open Life Sciences
Subjects:
alkaline phosphatase
direct elisa
glutathione s-transferase
his-tagged fusion protein
one-step western blot
scfv
Online Access:https://doi.org/10.1515/biol-2022-0521

Internet

https://doi.org/10.1515/biol-2022-0521

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