Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum
Abstract Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-022-02000-2 |
| _version_ | 1797958409705750528 |
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| author | Xihui Xie Jianchao Yang Hong Du Jichen Chen Edmond Sanganyado Yangmin Gong Hua Du Weizhou Chen Zhengyi Liu Xiaojuan Liu |
| author_facet | Xihui Xie Jianchao Yang Hong Du Jichen Chen Edmond Sanganyado Yangmin Gong Hua Du Weizhou Chen Zhengyi Liu Xiaojuan Liu |
| author_sort | Xihui Xie |
| collection | DOAJ |
| description | Abstract Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some special modifications, such as fucose-modification by fucosyltransferase (FucT). Three PtFucTs were annotated in the genome of P. tricornutum, PtFucT1 was located on the medial/trans-Golgi apparatus and PtFucT2-3 in the plastid stroma. Algal growth, biomass and photosynthesis efficiency were significantly inhibited in a knockout mutant of PtFucT1 (PtFucT1-KO). PtFucT1 played a role in non-core fucose modification of N-glycans. The knockout of PtFucT1 might affect the activity of PtGnTI in the complex and change the complex N-glycan to mannose type N-glycan. The study provided critical information for understanding the mechanism of protein N-glycosylation modification and using microalgae as an alternative ecofriendly cell factory to produce biopharmaceuticals. |
| first_indexed | 2024-04-11T00:19:39Z |
| format | Article |
| id | doaj.art-600cc41d748d44c28bad4ef749c50cce |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-11T00:19:39Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-600cc41d748d44c28bad4ef749c50cce2023-01-08T12:24:01ZengBMCMicrobial Cell Factories1475-28592023-01-0122111810.1186/s12934-022-02000-2Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutumXihui Xie0Jianchao Yang1Hong Du2Jichen Chen3Edmond Sanganyado4Yangmin Gong5Hua Du6Weizhou Chen7Zhengyi Liu8Xiaojuan Liu9Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityYantai Academy of Agricultural SciencesGuangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityGuangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityGuangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityGuangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityGuangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityGuangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityYantai Institute of Coastal Zone Research, Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou UniversityAbstract Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some special modifications, such as fucose-modification by fucosyltransferase (FucT). Three PtFucTs were annotated in the genome of P. tricornutum, PtFucT1 was located on the medial/trans-Golgi apparatus and PtFucT2-3 in the plastid stroma. Algal growth, biomass and photosynthesis efficiency were significantly inhibited in a knockout mutant of PtFucT1 (PtFucT1-KO). PtFucT1 played a role in non-core fucose modification of N-glycans. The knockout of PtFucT1 might affect the activity of PtGnTI in the complex and change the complex N-glycan to mannose type N-glycan. The study provided critical information for understanding the mechanism of protein N-glycosylation modification and using microalgae as an alternative ecofriendly cell factory to produce biopharmaceuticals.https://doi.org/10.1186/s12934-022-02000-2Phaeodactylum tricornutumGlycoproteinFucosyltransferaseN-glycanMicrobial cell factory |
| spellingShingle | Xihui Xie Jianchao Yang Hong Du Jichen Chen Edmond Sanganyado Yangmin Gong Hua Du Weizhou Chen Zhengyi Liu Xiaojuan Liu Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum Microbial Cell Factories Phaeodactylum tricornutum Glycoprotein Fucosyltransferase N-glycan Microbial cell factory |
| title | Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum |
| title_full | Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum |
| title_fullStr | Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum |
| title_full_unstemmed | Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum |
| title_short | Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum |
| title_sort | golgi fucosyltransferase 1 reveals its important role in α 1 4 fucose modification of n glycan in crispr cas9 diatom phaeodactylum tricornutum |
| topic | Phaeodactylum tricornutum Glycoprotein Fucosyltransferase N-glycan Microbial cell factory |
| url | https://doi.org/10.1186/s12934-022-02000-2 |
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