Protocol for localized macrophage stimulation with small-molecule TLR agonist via fluidic force microscopy

Protocol for localized macrophage stimulation with small-molecule TLR agonist via fluidic force microscopy

Summary: Here, we present a protocol to deliver nanoliter volumes of Toll-like receptor (TLR) agonist onto a culture of nuclear factor κB (NF-κB) reporter macrophages using fluidic force microscopy and a micron-scale probe. We describe steps for quantifying the dose of agonist by modeling their diff...

Full description

Bibliographic Details
Main Authors: Elizabeth J. Mulder, Brittany Moser, Jennifer Delgado, Rachel Steinhardt, Aaron P. Esser-Kahn
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Biophysics
Atomic Force Microscopy
AFM
Cell Biology
Cell culture
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000388

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724000388

Similar Items