The endogenous Mtv8 locus and the immunoglobulin repertoire
The vast diversity of mammalian adaptive antigen receptors allows for robust and efficient immune responses against a wide number of pathogens. The antigen receptor repertoire is built during the recombination of B and T cell receptor (BCR, TCR) loci and hypermutation of BCR loci. V(D)J recombinatio...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1345467/full |
| _version_ | 1827329670312361984 |
|---|---|
| author | Helen A. Beilinson Steven A. Erickson Tatyana Golovkina Tatyana Golovkina Tatyana Golovkina Tatyana Golovkina |
| author_facet | Helen A. Beilinson Steven A. Erickson Tatyana Golovkina Tatyana Golovkina Tatyana Golovkina Tatyana Golovkina |
| author_sort | Helen A. Beilinson |
| collection | DOAJ |
| description | The vast diversity of mammalian adaptive antigen receptors allows for robust and efficient immune responses against a wide number of pathogens. The antigen receptor repertoire is built during the recombination of B and T cell receptor (BCR, TCR) loci and hypermutation of BCR loci. V(D)J recombination rearranges these antigen receptor loci, which are organized as an array of separate V, (D), and J gene segments. Transcription activation at the recombining locus leads to changes in the local three-dimensional architecture, which subsequently contributes to which gene segments are utilized for recombination. The endogenous retrovirus (ERV) mouse mammary tumor provirus 8 (Mtv8) resides on mouse chromosome 6 interposed within the large array of light chain kappa V gene segments. As ERVs contribute to changes in genomic architecture by driving high levels of transcription of neighboring genes, it was suggested that Mtv8 could influence the BCR repertoire. We generated Mtv8-deficient mice to determine if the ERV influences V(D)J recombination to test this possibility. We find that Mtv8 does not influence the BCR repertoire. |
| first_indexed | 2024-03-07T15:44:27Z |
| format | Article |
| id | doaj.art-601882d9b48f4210b5bcabc749d86146 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-07T15:44:27Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-601882d9b48f4210b5bcabc749d861462024-03-05T04:59:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13454671345467The endogenous Mtv8 locus and the immunoglobulin repertoireHelen A. Beilinson0Steven A. Erickson1Tatyana Golovkina2Tatyana Golovkina3Tatyana Golovkina4Tatyana Golovkina5Department of Microbiology, University of Chicago, Chicago, IL, United StatesDepartment of Immunobiology, Yale University, New Haven, CT, United StatesDepartment of Microbiology, University of Chicago, Chicago, IL, United StatesCommittee on Microbiology, University of Chicago, Chicago, IL, United StatesCommittee on Immunology, University of Chicago, Chicago, IL, United StatesCommittee on Genetics, Genomics and System Biology, University of Chicago, Chicago, IL, United StatesThe vast diversity of mammalian adaptive antigen receptors allows for robust and efficient immune responses against a wide number of pathogens. The antigen receptor repertoire is built during the recombination of B and T cell receptor (BCR, TCR) loci and hypermutation of BCR loci. V(D)J recombination rearranges these antigen receptor loci, which are organized as an array of separate V, (D), and J gene segments. Transcription activation at the recombining locus leads to changes in the local three-dimensional architecture, which subsequently contributes to which gene segments are utilized for recombination. The endogenous retrovirus (ERV) mouse mammary tumor provirus 8 (Mtv8) resides on mouse chromosome 6 interposed within the large array of light chain kappa V gene segments. As ERVs contribute to changes in genomic architecture by driving high levels of transcription of neighboring genes, it was suggested that Mtv8 could influence the BCR repertoire. We generated Mtv8-deficient mice to determine if the ERV influences V(D)J recombination to test this possibility. We find that Mtv8 does not influence the BCR repertoire.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1345467/fullendogenous retrovirusimmunoglobulin repertoireV(D)J recombinationMTVB cell receptor (BCR) repertoire |
| spellingShingle | Helen A. Beilinson Steven A. Erickson Tatyana Golovkina Tatyana Golovkina Tatyana Golovkina Tatyana Golovkina The endogenous Mtv8 locus and the immunoglobulin repertoire Frontiers in Immunology endogenous retrovirus immunoglobulin repertoire V(D)J recombination MTV B cell receptor (BCR) repertoire |
| title | The endogenous Mtv8 locus and the immunoglobulin repertoire |
| title_full | The endogenous Mtv8 locus and the immunoglobulin repertoire |
| title_fullStr | The endogenous Mtv8 locus and the immunoglobulin repertoire |
| title_full_unstemmed | The endogenous Mtv8 locus and the immunoglobulin repertoire |
| title_short | The endogenous Mtv8 locus and the immunoglobulin repertoire |
| title_sort | endogenous mtv8 locus and the immunoglobulin repertoire |
| topic | endogenous retrovirus immunoglobulin repertoire V(D)J recombination MTV B cell receptor (BCR) repertoire |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1345467/full |
| work_keys_str_mv | AT helenabeilinson theendogenousmtv8locusandtheimmunoglobulinrepertoire AT stevenaerickson theendogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina theendogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina theendogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina theendogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina theendogenousmtv8locusandtheimmunoglobulinrepertoire AT helenabeilinson endogenousmtv8locusandtheimmunoglobulinrepertoire AT stevenaerickson endogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina endogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina endogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina endogenousmtv8locusandtheimmunoglobulinrepertoire AT tatyanagolovkina endogenousmtv8locusandtheimmunoglobulinrepertoire |