Two-photon microscopy with diffractive optical elements and spatial light modulators

Two-photon microscopy is often performed at slow frame rates, due to the need to serially scan all points in a field of view with a single laser beam. To overcome this problem, we have developed two optical methods that split and multiplex a laser beam across the sample. In the first method a diffra...

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Bibliographic Details
Main Authors: Brendon O Watson, Volodymyr Nikolenko, Roberto Araya, Darcy S Peterka, Alan Woodruff, Rafael Yuste
Format: Article
Language:English
Published: Frontiers Media S.A. 2010-09-01
Series:Frontiers in Neuroscience
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fnins.2010.00029/full