Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes
Abstract Background The three-finger proteins are a collection of disulfide bond rich proteins of great biomedical interests. Scalable recombinant expression and purification of bioactive three-finger proteins is quite difficult. Results We introduce a working pipeline for expression, purification a...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2024-02-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12934-024-02316-1 |