Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes

Abstract Background The three-finger proteins are a collection of disulfide bond rich proteins of great biomedical interests. Scalable recombinant expression and purification of bioactive three-finger proteins is quite difficult. Results We introduce a working pipeline for expression, purification a...

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Bibliographic Details
Main Authors: Jiang Xu, Xiao Lei, Ao Li, Jun Li, Shuxing Li, Lin Chen
Format: Article
Language:English
Published: BMC 2024-02-01
Series:Microbial Cell Factories
Subjects:
Online Access:https://doi.org/10.1186/s12934-024-02316-1